Construction Of Retrovirus Vector Of Focal Adhesion Kinase MRNA Cleaving Ribozyme Gene And Its Effects On The Gastric Cancer Cells

Object: To investigate FAK mRNA cleaving ribozyme gene effects on thegastric cancer cells of inhibiting Proliferation and promoting apoptosis so as toprovide the experimental data and academic foundations for the gene therapytargeting at FAKMethods: A hammerhead ribozyme cDNA targeting at FAK was synthesized,and recombinated into retroviral vector pLXSN forming pLRZXSN recon.Bylipofectin mediated DNA transfection technique,pLRZXSN was introduced intoSGC-7901 cells . The variation of FAK expression was detected through versetranscription-polymerase chain(RT-PCR) and immuocytochemistry staining. Theeffects of the ribozyme on the growth of K562 cells and apoptosis were studied byusing MTT assays and in situ cell apotosis detection(TUNEL).Results: The changes of SGC-7901 cells transfected with pLRZXSN arefollowing:â‘ The expression of FAKmRNA and the FAK protein was decreasedsharply in SGC-7901 cells,respectively.â‘¡we detected that SGC-7901 cells'proliferation was inhabited By MTT and apoptosion with 17.5 % positive rate by TUNEL.Conclusion: The ribozyme was capable of inhibiting the proliferation of K562cells and inducing the cell apoptosis.