Study On Modification Of Poly(Lactide) Film By Low-temperature Plasma And Its Cell Affinity

Polylactic Acid (PLA) is one of the synthetic polymer materials which is widely used in the research and application of tissue engineering. But because of its bad surface hydrophilicity and absence of natural molecule acting point, its application as biomaterials was limited.In this research, the NH₃/O₂ plasma and plasma grafting after Ar plasma treatment were used to modify its surface in order to improve its hydrophilicity, and discussed the affection on the amino density of material surface, the grafting density of PVP, the surface form before and after treatment, the change of contact-angle by different treatment conditions and make research on the cell affinity of modified materials.From the acid orange 7 coloration method, the different treatment condition would affect the amino density, and the surface contact-angle of materials. From the quality losing density and SEM observed, the surface of materials would be eroded by the treatment of plasma. The ATIR proved that PVP was grafted on the surface of material by plasma grafting. And its hydrophilicity was improved too, which its surface character was stabilization after grafting. The maximum adsorption of protein was on the surface treated by NH3 plasma from the observation of BSA adsorption; and the 3T3 fibroblast cells culture appeared that the cells were growing best on the surface treated by the NH₃/O₂ plasma, and the cell affinity was improved in a certain extent on the surface treated by the plasma grafting.