The Expression Of Cyclin E And Its Relation With P53 And Amplification Of CCNE In Endometrial Carcinoma

Objective: 1.To discuss on the relationship of the expression of cyclin E and p53 with the genesis, clinicopathological parameters of endometrial carcinoma (EC). 2.To discuss on the relationship among the expression of cyclin E and CCNE amplification in endometrial carcinoma.Methods: A total of 43 specimens of EC, 28 specimens of endometrial hyperplasia and 10 specimens of normal endometrium were evaluated for the expression of cyclin E and p53 by immunihistochemical S-P assay. Subsequently, a specifically and sensitively hybridization in situ was used to detect the amplification of CCNE in paraffin-embedded sections of 40 cases of endometrial carcinoma. All the patients were not treated by chemotherapy, radiotherapy and sex hormone therapy before operation.Results: 1.The positive immunostaining rate of cyclin E in EC (53.49%) was significantly higher than that in normal endometriums (0%) and endometrial hyperplasia (7.14%); and the differences was statistically significant between carcinoma and atypical hyperplasia (P<0.05). Cyclin E overexpression was associated with the histological type of EC, being more frequent in non-endometrioid endometrial carcinomas (NEECs, 5 out of 5 case) than in ECs (18 out of 38, 47.37%) (P=0.035). With the increasing of histological grade from G1 to G3 of EC, the positive rates of cyclin E were 21.05%, 66.67% and 4/4 respectively; and the difference was statistically significant between G1 and G2 or G3 (P<0.05). The expression of cyclin E significantly correlated with myometrial invasion: The expression of cyclin E in EC with deep myometrial invasion was higher than those without myometrial invasion (P<0.01). 2. The expression of p53 correlated with histological type, grade and clinical stage (P<0.05), but not withmyometrial invasion, lymph node metastasis and menopause. 3. There was an association between cyclin E overexpression and p53 positivity in EC (P<0.05). 4. CCNE amplification was found in seven (17.5%) tumours and there was a significant association between gene amplificant and cyclin E expression, since all seven tumours overexpressed cyclin E (P<0.05). There was also a statistically significant association between CCNE amplification and the histological type of the lesion, since four (57.14%) of the seven cases with amplification were NEECs (P<0.01).Conclusion: These results suggest that the aberrant expression of cyclin E and p53 are related to the occurrence and development of EC, coabnormal expression of these two genes may act as a newfashioned predictor of poorer prognosis of EC; The association between gene amplificant and cyclin E expression may provide evidence for genie diagnosis and treatment of EC.