The Protective Effect Of Vascular Endothelial Growth Factor Gene In Injured Renal Artery Intima Of Dog

Objective: To establish the making method of renal artery intima injury model of the dog and the specific operation procedure of this method. To find the effective method of gene transfer in vascular system through balloon coated gene. To study vascular endothelial growth factor(VEGF) gene expression in dog renal artery intima and its therapeutic efficacy to injured renal artery intima.Methods: We produced an eukaryotic expression plasmid of pcD2/VEGF121 and pcD2 plasmid as a large scale by the technique of molecular biology, identified them by agarose gel electrophoresis and assayed the sequence of DNA. The two sides of renal arteries of 14 dogs were randomly divided into 2 group: therapy side and control side. Renal arteriography of 14 dogs were performed and then the PTCA balloon was sent into renal arteries and injured the renal artery intima by compression of balloon. The balloon coated pcD2/VEGF121 plasmid and pcD2 plasmid was respectively sent into therapy side and control side of renal arteries and transfer the gene to renal artery intima by compression of balloon for 5 minutes. The dogs were bred after operation. Repeated renal arteriography were performed respectively at 1 week, 6 week and 10 week after operation and we observed the changes of renal artery intima, the expression of VEGF protein and ultrastructure of renal artery by HE staining and immunohistochemistry and electron microscope.Results: We could produce 3.0mg pcD2/VEGF121 plasmid or pcD2 plasmid from 400ml fluid of bacteria. A260/A280 of plasmid was 1.8-2.0 and objective gene band of 564 bp...