| Objective To explore the pathogenic and clinical roles of chemokines and their receptors in systemic lupus erythematosus(SLE) by detecting the serum concentration of MIP-1α, MIP-1β, RANTES and the expression rate of their receptors CCR1, CCR3, CCR5 on CD4+T cells.Methods Thirty seven new-onset SLE patients (including eighteen lupus nephritis patients and nineteen non-lupus nephritis patients) and twenty normal controls were selected. Clinical data and blood were collected and sandwich ELISA was used to examined the serum concentrations of MlP-1α, MIP-1β, RANTES, IFN- γ and IL-4 before and six weeks after treatment in SLE patients. Eighteen new-onset SLE patients (including ten lupus nephritis patients and eight non-lupus nephritis patients) and ten normal controls were selected randomly. Flow cytometry was used to detect the expression rate of CCR1, CCR3, CCR5 on CD4+T cells. The interaction and association of serum concentration of chemokines and expression level of their receptors, with SLE activity and organ damage were analyzed.Results 1. Serum concentration of MlP-1α and MIP-1β in SLE group were significantly higher than that in controls(P<0.001, P=0.003). There was no significant difference between serum concentration of RANTES in SLE group and that in controls(P=0.327). The expression rates of CCR1 and CCR5 on CD4+T cells in new-onset SLE patients were significantly lower than that in controls (P<0.001, P<0.001) and the ratio of CD4+CCR3+/CD4+CCR5+ was significantly higher than that in controls (PO.001). There was no difference between SLE patients and controls about the expression rate of CCR3 (P=0.596). 2. Serum concentration of MlP-la in lupus nephritis group and non-lupus nephritis group was markedly higher than thosein control(P<0.001, P=0.004),But there was no significant difference between lupus nephritis group and non-lupus nephritis group (P=0.416); Serum concentration of MIP-ip in non-lupus nephritis group was markedly higher than those in control (P=0.012), and there was no significant difference between lupus nephritis group and non-lupus nephritis group(P=0.040);The case was the same to that between lupus nephritis group and normal controls(P=0.061).The expression rate of CCRl and CCR5 on CD4+T cells were significantly lower than that in controls(P<0.001, PO.001), and the ratio of CD4+CCR3+/CD4+CCR5+ in lupus nephritis group was significantly higher than those in controls (P=0.010). The case was the same to that in non-lupus nephritis group compared with controls (PO.001, PO.001, PO.001). But there were no marked differences about expression rate of CCRl and CCR5 and ratio of CD4+CCR3+/CD4+CCR5+ between lupus nephritis group and non-lupus nephritis group(P=0.643, P=0.761, P=0.121). 3. After treatment, serum concentration of MlP-la and MIP-lp in SLE group decreased significantly (P=0.001, P=0.008). But the changes was not significant in serum concentration of RANTES (P=0.711). In non-lupus nephritis group, serum concentration of MIP-1(3 decreased markedly after treatment (P=0.035). There were no significant difference about the serum concentrations of MlP-la and RANTES (P=0.078, P=0.450). In lupus nephritis group, serum concentration of MlP-la decreased significantly after treatment (P=0.003), while the changes of MIP-ip and RANTES were not significant (PO.136, P=0.279). The expression rate of CCRl on CD4+T cells increased significantly in new-onset SLE patients (P=0.022). But the difference of the expression rate of CCR3 and CCR5 before and after treatment and the ratio of CD4+CCR3+/CD4+CCR5+ were not significant (P=0.496, P=0.065, P=0.129). 4. Bivariate correlation analysis indicated that MlP-la was positively related to MIP-ip (r=0.609, PO.001), while the expression rate of CCRl on CD4+T cells was negatively related to MlP-la and IFN-Y (r=-0.525, P=0.017; r=-0.442, P=0.045). The expression rate of CCR5 was negatively related to IFN-y (r=-0.645, P=0.001).The result of partial correlation analysis showed that MIP-ip was positively related to RANTES (r=0.4344,P=0.0l0;... |
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