Studies On The SOX4 Gene Mutation In Colorectal Cancer Cells

PartⅠ: Reviews 1. Tumor`s development involves many kinds of factors and through several stages. The essence of the tumor is molecular diease. It is the result of the abnormal changes in genes. Colorectal cancer is a kind of usual malignant tumor. The origination of this tumor involves 3 kinds of gene mutations, namely, oncogene,suppressor gene and mismatch repair gene (MMR) . In this article , we reviewed the studies on oncogene,suppressor gene and mismatch repair gene in colorectal cancer. 2. The SOX gene family of transcription factors highly related to the SRY gene . They are characterized by the presece of a HMG domain involved in various developmental processes . The encoded proteins are capable of binding to DNA in a sequence-specific manner . In human , the deletion or mutation of SOX proteins results in developmental defects and congenital disease . It has been shown that mutations in the human SOX9 gene cause campomelic dysplasia(CD) and autosomal sex reversal, SOX10 mutation cause Waardenburg syndrome(WS) , and so on . This paper reviewed the progress of the relation between the mutation of SOX gene and disease occurrence in recent years . Part Ⅱ: Studies 1. Using PCR—SSCP technique , we analysisd SOX4 gene in 13 colorectal cancer tissues and the normal tissue beside cancer . we found mutations in 5 cases,but it wasn`t found in the normal tissues. 2. SOX gene is a new kind of development regulation gene family based on SRY gene as the basic gene member . The coding production of this gene is a kind of very important transcriptional regulation factor . Studies show that in the SOX gene family , SOX4 gene may influence the transcriptional regulation of it`s downsteam gene and cause the origination of the colorectal cancer . In this study , we cloned 5 cases of HMG–box of SOX4 gene which was found to have mutations through the PCR—SSCP check . After sequenceing , we found point mutations at 10541,10580,10476,10485,10518,10648 and 10666 bp in the HMG–box regions of SOX4 gene in 5 cases , but it was not found in normal tissue beside cancer. It suggests that the SOX4 gene mutation may be associated with the origination and development of the colorectal cancer. Our studies first reports the SOX4 gene mutation of the colorectal cancer tissues . And also provides molecular data for the study of the relations between the mutation of SOX gene and disease occurrence.