Proteomics Profile Changes In Cisplatin-treated Human Ovarian Cancer Cells

Objiectives: To compare the alterations in proteomes between cisplatin-treated and -untreated human ovarian cancer SKOV3 cells, and to explore the feasibility of proteomic technologies on research about chemotherapy-drug antitumor mechanisms.Methods: SKOV3 cells were exposed to cisplatin(6 μ g/ml) for 6 hours, then, 1)collected and solubilized and total proteins were extracted by lysis buffer; 2)two-dimensional electrophoresis was performed with the IPG readystnps as carriers; 3)gels were stained with Coomassie blue and changes were compared between treated- and untreated-groups,those spots with dominant differences were selected; 4)and the proteins in selected spots were identified by PMF and MS/MS analysis.Results: Two pieces of gel proteome patterns were achieved and 11 spots picked out. As MS analysis revealed, those increased proteins included tropomyosin family, actin family, triosephosphate isomerase family, HSP60,et al; and the decreased proteins included enolase family ,et al. Conclusion: Exposure of SKOV3 cells to cisplatin can induce expression changes in a few proteins, which are involved in energy metabolism, transformation, apoptosis and morphologic maintenance of cells. Methodologies based on 2-D electrophoresis and MS analysis, therefore, would have fine prospects in proteomic research about chemotherapy-drug antitumor mechanisms.