Effect Of Matrine On Proliferation And Apoptosis Of Rat Hepatic Stellate Cell In Vitro

Objective To investigate the effect of matrine on proliferation and apoptosis of Hepatic Stellate Cell in vitro.Methods (1)HSC line,rHSC-99 and hepacyte line,HL-7702 was incubated with matrine of different concertration of 0.125mg/ml , 0.25mg/ml, 0. 5mg/ml for 24h,cell proliferation was assessed by MTT colorimetric assay. (2)rHSC-99 was treated with matrine of different concertration of 0. 125mg/ml, 0.25mg/ml, 0.5mg/ml for 24h, the ealy stage apoptosis was detected with Annexin v-FITC/PI double labbled assay, DNA break was detected by TUNEL in situ assay, morphology of apoptosis was detected by transmission electron microscopy.Results Administration of 0.125-0.5mg/ml matrine could significantly inhibit HSC proliferation in dose-dependent manner (F=14.32, P<0.05) and promot hepacyte proliferation in dose-independent manner(F=86.69 , P<0.05).After treated with matrine at different concertration of 0.125, 0. 25, 0. 5mg/ml matrine for 24h, the apoptosis rate were5. 00%±0. 98% , 13. 6% ± 2.19% , 17. 2% ± 2.17%, respectively, which is significently higher than that of control group(0. 99%±0. 45, F=63.14, P<0.05). DNA break of matrine-treated cells was demonstrated by TUNEL in situ assay, Electron microscopy showed that nucleoli disappeared, chromatin condensted, ranked along inside of nucleonic membrane.Conclusion Matrine can inhibit HSC proliferation and induce apoptosis of HSC, which might be one of the mechanisms of matrine to counteract liver fibrosis.