| Ovarian cancer represents the most frequent cause of death for gynaecologic malignancies.In fact, after primary surgery,the overall 5-year survival is still very low,mainly because of lack of improvement in early diagnosis and particular aggressiveness of the disease.Nearly 80% of ovarian malignant tumours are of the epithelial type,with multiple genetic changes involved in their genesis and progression,including the activation of proto-oncogenes and inactivation of tumour suppressor genes.The FHIT(Fragile Histidine Triad)gene is a probable tumor suppressor gene,it's located at 3pl4.2 and encompasses the FRA3B fragile site and the breakpoint of the (3:8)translocation associated with familial renal cell carcinoma .The FHIT gene is expressed at low level in various human tissues.Whereas the high frequency loss and /or aberrant transcripts of FHIT has been reported in various human cancers, inactivation eventually lead to lack of normal anti-oncogene expression in tumor cells. Abnormal structure or expression of fhit gene in ovarian cancer have been reported abroad. Loss or aberrant expression of fhit protein was observed in the ovarian carcinoma but normal expression of FHIT protein wasobserved in benign ovarian tumour and normal ovarian tissue, FHIT protein is important suppressor gene in malignancies,the expression of the FHIT protein is related to histological grade of tumors and prognosis . It is being studied that abnormal expression of FHIT protein in ovarian cancers may be used to diagnose the patients in early stage and evaluate therapeutical effect and direct the clinical treatment.In this project ,the fhit protein is examinated in ovarian benign and malignant tumours.In order to indicate the location,distribution of the FHIT protein in ovarian cancer cells, and the relationship between the FHIT protein expression and the clinicopathological features.Moreover,the role of FHIT protein in the genesis and development of ovarian cancers and the relationships among FHIT protein,the clinicopathological manifestation and the prognosis.In addition,in order to provide the theoretics basis for the early diagnosis ,biological and genetic treatment on ovarian caners. 1.Methods:1.1 Expression of fhit protein was analysed by immunohistochemistry in paraffin embedded 37 ovarian carcinomas, 10 benign adenomas and 5 normal ovarian tissue, and the results were studied in correlation with clinicopathological characteristics of patients.1.2 Statistical treat: All experimental data from expression of FHIT protein and clinicopathological index were analyzed using Fisher's exact test of probabilities and chi-square test and processed by spss10.0 There was a statistical significance when P<0.05.2.ResuIts:2.1 The FHIT protein located in cytoplasm of the ovarian tissue, benign adenomas and the ovarian cancer cells.2.2 Strongly positive staining was observed in 10 benign adenomas and 5 normal ovarian.2.3 Loss or significantly reduced expression of FHIT protein was observed in 5/37(13.51%) ovarian carcinomas.2.4 The impaired expression of the FHIT protein was significantly related to high malignant potential. Including high histological grade, lymph node metastasis,and the size of residual tumor after operation(p<0.05) 3.ConcIusion:3.1 Loss of FHIT protein characterizes ovarian cancer.3.2 Expression of fhit protein in ovarian carcinomas was lower significantly than benign adenomas .This Indicate that the FHIT protein was significantly related to the genesis and development of ovarian cancers,and may become the mark of biology in state of an illness and early diagnosis in ovarian carcinomas.3.3 The expression of the FHIT protein was significantly related to high histological grade, lymph node metastasis,and the size of residual tumor after operation(p<0.05),Indicating that it is related to the genesis , development and prognosis in ovarian carcinomas. |
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