Telomerase Anti-hTR And Anti-hTERT Inhibit Cervical Cancer Hela Cells Growth

Objective: To investigate the proliferation inhibitory effect of telomerase anti-hTR and anti-hTERT alone and combination of them on cervical cancer Hela cells cultured in vitro and its mechanism.Methods : Cervical cancer Hela cells were transfected by sense-hTR, sense-hTERT, anti-hTR, anti-hTERT,anti-hTR+anti-hTERT with oligofectamineTM reagent and oligofectamine? alone. The proliferation activity of cervical cancer Hela cells were determined using methyl thiazolyl tetrazolium (MTT) assay .The activity of telomerase was tested by telomeric repeat amplification protocol - polymerase chain reaction -enzyme-linked immunosorbent assay (TRAP - PCR - ELISA) and telomeric repeat amplification protocol - polymerase chain reaction - Polyacrylamide gel electrocphoresis (TRAP - PCR -PAGE ) .Cell morphologies were observed by fluorescence microscope stained with acridine orange .Apoptosis and cell cycle were examined by flow cytometer method (FCM).Results : The proliferation inhibitory rates and apoptotic rates of Hela cells transfected by antisense oligonucleotides were significantly higher compared with those of nontreated Control, sense oligonucleotides and oligofectamineTM (P < 0. 01). The difference between anti-hTR and anti-hTERT wasn't significant ( P > 0.05 ). Yet the proliferation inhibitory rates and apoptotic rates of Hela cells transfected by anti-hTR + anti-hTERT were significantly higher compared with those of transfected by anti-hTR or anti-hTERT alone (P<0.01); meanwhile, telomerase activity was significantly decreased (P<0.01). Transfected by 0.2 μ mol/L antisense oligonucleotides after 3 d, proliferation inhibitory rates,relative telomerase activity (%...