Calcium Signal Of AD Model Cell

Alzheimer's Disease(AD) is a progressive and irreversible neurodegenerative disorderthat leads to cognitive, memory and behavioural impairments. The pathogenesis ofAlzheimer's Disease is complex, and involves many molecular, cellular and physiologicalpathologies. With the coming of aging society, the correspongding research attracts moreand more attention in the world. However, its pathogenic mechanism is still poorlyunderstood now. Calcium modulates many neural processes, including synaptic plasticityand apoptosis. It is irrefutable that calcium dysregulation is involved in theneurodegeneration of Alzheimer's Disease. Neurodegeneration that is induced byamyloid-βor tau protein is probably mediated by changes in calcium homeostasis.Moreover, mutations that cause familial Alzheimer's Disease have been linked tointracellular calcium signalling pathways. Destabilization of calcium signalling seems to be akey factor in the pathogenesis of Alzheimer's Disease, and drug design targeting this processmight be therapeutically promising.Using the image technology of confocal laser scanning microscope, we studied themechanism of the calcium dysregulation of the AD model cell s.Three aspects of work were designed and have performed in the research:In the first part of the dissertation, to test the feasibility of using N2a cell as model cellin AD calcium signal, we studied the morphological and functional differences between N2aand hippocampus neuron. After being treated by 10-5 uM RA(Retinioc Acid) for 7 days, N2acell could grow axon and form network .The result of K+ depolarization stimulationexperiment indicate that the Ca²⁺ wave of N2a cell was closed to hippocamp neron. Fromthese resulst, we can conform that using N2a cell as model cell in AD calcium signal isviable.In the second part of the dissertation, we employed the image technology of confocallaser scanning microscope to study the interaction between APP695(amyloid precusorprotein 695,APP695 ) mutation and calcium dysregulation. At present, calcium dysregulation...