The Role Of Estradiol In Regulatory Mechanisms Of T Cell Activation â…¡.The Role Of Estradiol In Cell-contact-dependent Regulatory Mechanism

Objective To investigate whether estradiol plays a role in cell-contact-dependent regulatory mechanism of T cell activation,we studied the role of E2 in regulating gene transcriptions of CTLA-4, ICOS, B7-1, B7-2 and B7h in vitro.Methods The spleen cells of normal female Balb/c mouse were activated by ConA.The cells were cultured with E2(100pg/ml or 50ng/ml) for 24h or 48h respectively.The spleen cells proliferation was measured by MTT assay, and the costimulatory molecules gene transcription was measured by RT-PCR.Results E2(100pg/ml,physical level) stimulated activated spleen cells proliferation;inhibited CTLA-4, ICOS, TGF-6 and IL-10 genes transcription;promoted B7-1 and B7-2 genestranscription.E2(50ng/ml,pregnant level) inhibited the proliferation of activated spleen cells; promoted CTLA-4, B7-1, IL-10 but inhibited B7-2 and TGF-6 genes transcription.Conclusions The data suggests that the effects of E2 on T cell activation is partially through its regulation on the costimulatory molecules.