| objection: 1:established appropriate animal model of hepatic metastasiscarcinoma and infused phosphorus–32 glass microspheres (32P-GMS) byintra-portal inject .2: explored the treatment efficacy of intro-portal infused Internalradioembolization of 32P-GMS.3:observed the side-effect of hepar function andthe renal function after intra-portal infused 32P-GMS.Material and method: we used 30 zealand rabbits and established hepaticmetastasis tumor model by intro-portal infused of VX2 carcinoma. randomizeddivided into three groups after 14 days, A group was control group that intro-portalinjected 1ml 50% glucose;B group was GMS group that injected the suspension ofGMS mixed with 1ml 50% glucose by intro-portal;C group was P-GMS group 32that injected the suspension of 32P-GMS mixed with 1ml 50% glucose byintro-portal, Alanine aminotransferase(ALT) ,Aspartate aminotransferase(AST),blood uric acid (BUN)and CR was checked post-treatment 1 day ,7daysand 14days, The experimental animals in each group were put into death in 14 daysafter injected drugs. The tumor tissue was gained and observed the charges ofpathologic structure with microscope and transmission electron microscope(TEM)to know clearly that radioactive internal radiation induced the lesion degree oftumor cells,Results: 1:the charges of tumor volume: C groups were observed that the tumorvolume significant smaller than A and B group, B group tumor volume is smallerthan A group, but not statistic significance.2: Alanine aminotransferase andAspartate aminotransferase level was obvious hoisted in post-treatment 14 daysthan pre-treatment in A,B,C groups. But not statistic significant change amongthree group in post-treatment 7 days and 14 days. Renal function (BUN,CR) notobvious change.3): Pathology observation in general: in the control group and theGSM group, carcinoma present nodosity in liver parenchyma, no peplos, gray fish,stereoplasm, affluent blood vessel . cut biggish carcinoma show jam fluid, somecavitates with irregular wall. The carcinoma volume and blood vessel density inthe 32P-GMS group is obviously decreased compared with the control group andthe GMS group.4)microscope observation: in the control group and the GSMgroup, multiple invasion cancer nest are displayed, no obviously bouncary with thenormal liver parenchyma, and show lymphocyte and plasmocyte infiltratinginterstitial; in the 32P-GMS group , tumor cell present punctiform and lamellarnecrosis, copious lymphocyte and plasmocyte infiltrating zone of necrosis.5)transmission electron microscope observation: under transmission electronmicroscope, four kinds of cells are showed: early dead cell, degenerative cell,survival tumor cell. In in the control group and the GSM group, survival tumor cellis the preponderant cell and it's percentage is 95%. dead cell is scanty. in the32P-GMS group , dead cell reach 50%.Conclusion: 1).rabbit VX2 hepatic metastatic carcinoma model possess thesefeatures of short growth cycle, high achievement ratio, steady model. It have somesimilar aspects with human hepatic metastasis carcinoma in pathological displayand process and metastatic circumstance. So it is a kind of ideal animal modal toresearch hepatic metastasis carcinoma. 2).throught the method of portal veinperfusion, 32P-GMS has the inhibition and lethal effect to the hepatic metastasiscarcinoma growth, moreover, it has not impact the hepar function and the renalfunction. So it is a kind of safe and effective therapeutic method.3.when 32P-GMStreat hepatic metastasis carcinoma, effective therapy function is the β-ray of 32Pnot but the inhibition of glass microspheres embolism. Hepatic portal instillation of32P-GMS appears to be safe and effective for hepatic metastasis carcinoma. |
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