| Background and purpose:It finds that central nervous system hasthe ability of structural and functional reorganization,There is highlyplasticity in central nervous system after injury. Axon sprouting andsynapse formation and changes in structure and function of synapse areimportant material basis of brain functional reorganization. The treatmentof limb electrical stimulation can promote functional recovery. Traditionalrehabilitation trainings often concentrate in hemiparetic limbs of strokepatients , there were seldom reports about bilateral rehabilitation trainings.We investigated the effect of different kinds of limb electrical stimulationon axon sprouting and expression of synaptophysin and synaptic interfacestructure following cerebral infarction in rats and discussed the materialbasis for the clinical treatment of electrical stimulation to functionalrecovery. Methods:(1)Model making and group dividing :180 adult malerats(250-300g), A model of the middle cerebral artery occlusion in rats wasperformed with the intraluminal filament occlusion. The success of modelare determined by 5 degree. The 2 and 3 degree ,138 rats were selected inall experiments and were divided randomly into controlled group ,ipsilateral stimulation group and bilateral stimulation group.(2)Electricalstimulation :The different kinds of stimulation was performed in theforelimb at the level of wrist of the subjects with 6805-Celectro-acupuncture apparatus( 4-5V, 0.5ms, 3Hz, 30min per day)postoperatively. (3) Behavioral assessments: Behavioural analysis wasperformed on all rats using beam walking test which shows functionalrecovery on days 7, 14 and 28 postoperatively. (4) Synaptophysinimmunostaining and silver staining:12 rats were take from each group andperfused with polyoxymethylene, then the brains were removed and fixed .The level of optic chiasma was selected for paraffin sections of 6um ,Sections were immunostained with a monoclonal antibody tosynaptophysin. Also the sections were selected for silver staining at timepoint 28 days . (5) For electron microscopy: 10 rats were selected fromeach group and perfused at 28 days, the brains were removed , fixed ,soaked, embedded and made into Ultrathin sections. Ultrathin sectionswere cut and stained with uranyl acetate and lead citrate, and examinedwith a Philips CM10 transmission electron microscope. (6) Imagecollection and data processing: Olympus BX51 microphotograph systemand Image-Pro Plus analysis software were used to collect image and dealwith data. (7) Statistical analysis: SPASS12.0 was used to analysis data. Allvalues were represented by mean±SD. The data were analyzed with the useof ANOVA for multiple comparisons and Student's t test for comparisonbetween two groups with P<.05 set as the level of confidence. Results:(1)The effect of different electrical stimulation on functionalrecovery of movement: Analysis of behavioural tests revealed significantdifferences in recovery among each group. The functional recovery inControlled groups rats are slower, electrical stimulation groups rats showedsignificant improvements of functional recovery(p>.05), bilateralstimulation groups were significant improved compared with the ipsilateralstimulation groups at 14th, 28th days.(2) The effect of different electricalstimulation on axon sprouting: axon density was different in every groups.Compared with control group(37.20±5.79), electrical stimulation groupsshowed significant increased in axon density. bilateral stimulation groupalso showed significant changed compared with the ipsilateral stimulationgroup.(3) The synaptophysin demonstrated statistically significantincreases at 14 day [(21.05±2.46),(30.34±3.18),(32.48±3.81)], 28 day[(27.11±3.17), ( 45.15±3.35),( 48.91±3.96)] in the region near theinfarction. there was a significantly higher increase in synaptophysinimmunoreactivity in electric stimulation groups compared with controlledgroups at 14th, 28th days(p>.05), and the synaptophysin reaction productwas statistically significant increased in the bilateral stimulation groupcompared... |
PDF Full Text Request