Effect Of BFGF And EGF On Multiplication Of Neural Stem Cells From Rat Embryo

Objective: To explore the effect of bFGF and EGF on multiplication of neuralstem cells from rat embryo. Methods : Striatum tissue was dissected out from rat embryo in vitro and madeinto single-cell suspension, which was cultured in DMEM/F12 with thepresence of bFGF, EGF and B27. The characteristics of neural stem cellswere detected by phase contrast microscopy and immunofluorescence. Thegrowth of neural stem cells was observed in different densities of EGF orbFGF. The growth of neural stem cells was observed in different densitiesof EGF and bFGF. Results : Neural stem cells can be cultured in rat embryo in vitro. Multiplicationof neural stem cell from rat embryo can be accelerated by bFGF andEGF .Furthermore, bFGF have cooperate with EGF in this reaction.bFGF , EGF and bFGF+EGF can accelerate the multiplication of neuralstem cells from rat embryo at 10~20 ng·mL-1 ,20~30 ng·mL-1 and 20~30ng·mL-1 respectively. Conclusions : We have found the most suitable density of EFG or bFGF for theculture of the neural stem cells. Multiplication of neural stem cells from ratembryo can be accelerated by bFGF and EGF . Furthermore, bFGF andEGF have a coordination effect in this reaction.