The Effect Of Ploysaccharide Sulfate On Insulin Resistance In Fat-fed, Streptozotocin-Treated Rats

AIMTo investigate the therapeutic efficiency of ploysaccharide sulfate ( PSS) on glycometabolism in fat - fed, streptozotocin - treated diabetic rats ( Fat - fed/ STZ rats).METHODSThe rat model of type 2 diabetes was established. Male Wistar rats adapted surrounding for a week, then they randomly divided into normal control group ( NC group) and model groups that were randomly divided into 5 groups: diabetes control group (DC group) , PSS groups (30 mg · kg^-1 ,90 mg · kg^-1 , 180 mg · kg^-1) and metformin group (200 mg · kg^-1). NC rats were fed conventional chow for seven weeks and then injected with vehicle of 0.1mol/L citric acid buffer ( pH 4.5) ; while model rats were fed high - fat diet (30% of calories as fat) for seven weeks and then injected with streptozotocin (STZ, 20mg kg^-1, via the caudal vein once). After two weeks of STZ treatment, All of groups were administered daily by gavage for a months. NC group and MC group were administered with 0. 1mol/L citric acid buffer. Then the effects of PSS on fast blood glucose, insulin and lipids concentrations in fat - fed, strepotozotocin - treated diabetic rats was studied.