| Inhibition effects on proliferation of cancer cells is a kind of biological effect of nano-particles himself, and it had been studies by the Biomedical Materials and Engineering Research Center of Wuhan University of Technology for many years. In this study, different component nano-particles, HAP, Sr-HAP1, Sr-HAP2 and TiO2, were prepared by the homogeneous precipitation, and different size HAP particles, HAP1, HAP2, HAP3 and HAP4, were prepared by different experiment condition controlled. According to the character of nano-particles that was distinct inhibition on proliferation of Bel-7402 hepatocellular carcinoma cells and mild inhibition on L-02 hepatocytes, HAP1 nano-particles which average size was less-than 100nm and were well distributed, and could be metabolized in vivo, were selected as the material basis for future research. Method of growth curve and cell clone were used to detected the inhibition on proliferation and rate of cell clone formed of Bel-7402 hepatocellular carcinoma cells treated with different concentration HAP1 nano-particles. Morphology change was observed through microscope after treated with different concentration HAP1 nano-particles. Laser scanning confocal microscope (LSCM) was used to research endoplastimic reticulum (ER) in Bel-7402 hepatocellular carcinoma cells qualitatively and quantitatively after treated with different concentration HAP1 nano-particles. The conclusions are received. 1. Proliferation of Bel-7402 hepatocellular carcinoma cells is inhibited treated with HAP1 nano-particles, and the inhibition is dose-dependent and time-dependent in range of concentration. 2. Rate of cell clone formed is descent after treated with HAP1 nano-particles, rate of cell clong formed of control group is 97.78 ± 1.50%, and the rate of different concentration HAP1 nano-particles, HAP1-1 group, HAP1-2 group and HAP1-3 group, are 73.11 ± 1.67%, 40.89±2.16% (p<0.01, compared with control group) and 15.44±2.50% (p<0.01, compared with control group) respectively. Radiu and area of cell clone are distinct decreased (p<0.01) compared with control group. 3. Vaculoes are observed in cells after treated with different concentration HAP1 nano-particles. Then many vacuoles are fused, which perhaps causes Bel-7402 hepatocellular carcinoma cellsdeath. 4. Quantity and distribution of ER in Bel-7402 hepatocellular carcinoma cells are changed treated with different concentration HAP1 nano-particles. Number of fluorescence intension and average fluorescence of treated groups are distinct decreased compared with control group. Formation and function of ER are varied after treated with HAP1 nano-particles, which affect cell transcription, inhibit protein synthesis, block cell metabolism, change the process of cell differentiation and made cells death. |
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