| Objective:To investigate if fresh allogeneic osteochondral transplantation, combined with basic fibroblast growth factor, could accelerate the formation of new articular cartilage and improve the rate of success in the repair of the defect of articular cartilage, when they were adopted to repair the defect of articular cartilage in the knee of rabbit.Methods: .48 rabbits were used in the experiment ,both knee joints of them were considered, so there were 96 experimental joints in the experiment .They were randomly divided into 4 groups: fresh allogeneic osteochondral transplantation was migrated into the defect of articular cartilage in the knee of rabbit in A group; basic fibroblast growth factor was injected into the defect in B group; fresh allogeneic osteochondral transplantation was combined with basic fibroblast growth factor in C group; the fourth was the control group . The experiment was done in asepsis condition.The model of the defect of articular cartilage was made by special round saw between condyles of femur. The obtained osteochondral mass was used for transplantation each other. General observation, X-ray examination , magnetism resonance imagine , histology examination and immunohistochemistry examination were performed at 4th week,8th week,12th week after the operation.Results: 1 general observation The activity of rabbits were not evidently abnormity after operation. All incision were healed very well.The position of grafted articular cartilage was basically resumed in C group , and its surface was still lubricity .A little depression was found ,but no flexible in A group. A depression was also found in B group, and there were a little adherence in articular capsule, Othermore , hyperosteogeny were formed in these knee joints .A big defect in the knee was still exist in the control group, the defect were filled with fibroagents , and there were adherence in articular capsule.2 X-ray examination There were no evidently defect in the position of grafted articular cartilage in C group, and its surface was smooth .The defect in B group was bigger than that in A group ,and the hyperosteogeny were formed in B group, The defect was not resumed in the control group .3 Magnetism resonance imagine The thickness of new cartilage was about 92%(11/12)similar to the perimeter articular cartilage in C group . Among them, surface of the articular cartilage,which was smooth ,was about 60%,and 40% was irregularity . The thickness of new cartilage was about 63%(5/8)similar to the perimeter articular cartilage in A group , 50% was smooth ,and 50% was irregularity .It was about 25%(2/8) similar to the perimeter in B group, 20% was smooth ,and 80% was irregularity . The defect in the control group was filled with soft tissue , and cartilage proliferation was not found .The subchondral cyst was not also performed in all groups .4 Histology examination The new cartilage in C group was bonded integratedly with the perimeter articular cartilage ,a lot of hyaline chondrocyte and cartilage lacuna might be seen in these tissues . Hyaline chondrocyte might also be seen in A group, but less than in C group . The defect in B group was composed of hyaline chondrocyte and fibrocartilago tissues . The defect in the control group was filled with fibrous tissues ,and a little chondrocyte might be seen. 5 Immunohistochemistry examination Immunohistochemistry results of type â…¡ collagen were positive in A,B,C groups, but negative in D group .6 Histology score there were significant deviation between the C group and the others.(p<0.05) 7 to count cartilage cell by image analyzer Significant deviation was found between the C group and the others.(p<0.05), so there were statistical significance.Conclusion: Fresh allogeneic osteochondral transplantation, combined with basic fibroblast growth factor, could accelerate the formation of new articular cartilage and improve the rate of success... |
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