| Selenium is one of essential trace element to animal and human nutrition, which enhancing immunity, resisting cancer and antioxidating, postponing aging, antagonizing toxicity of some heavy metals, preventing or curing parochial epidemic and so on. With the development of technology, the research on selenium has been paid more and more attention to the biological significance of selenium for all of living thing. Area of deficiency selenium is seventy percent of china. Recent research about parochial epidemic showed that the deficiency selenium is associated with occurrence of tumour and selenium has been a kind of effective trace element for preventing tumor. Therefore, the research about mechanism of selenium for preventing tumor has become one of important project worldwide.Selenium-enriched malt was made by Lab of the nutritional and metabolic disorders. Se-enriched malt was used as selenium sources in rat diet, in order to observe effects of the antioxidant abilities, hepatic function and anti-hepatocarcinogenesis which was induced by diethynitrosamine (DENA). Two parts were included in this research work.EXPERIMENT IThe effects of selenium in selenium-enriched malt at different levels on antioxidant abilities, and on hepatocarcinogenesis induced by diethynitrosamine (DENA) in rat were observed. One hundred and forty-seven male healthy and weighted 120-150g SD rats were randomly divided into three groups. Thirty rats in group I were fed with 0.1 mg selenium from selenium-enriched malt in per kilogram diet, designed as normal control; eighty-five rats in group II were fed with 0.1 mg selenium from selenium-enriched malt in per kilogram diet, designed as hepatocarcinogenesis control; thirty-two rats in group III were fed with 0.3 mg selenium from selenium-enriched malt in per kilogram diet,designed as selenium control.The rats in group II and III drinked the water with 100 mg L DENA. During the following days, GSH-Px in whole blood, SOD and MDA in serum of each rat from three groups weremonitored and recorded. The data showed that supplemented with 0.3 mg selenium in per kilogram diet could increase the contents of GSH-Px in whole blood and SOD in serum , decrease MDA in serum, which indicated that the 0.3 mg selenium in selenium-enriched malt could enhance the anti-oxidation capacity.EXPERIMENT IIThe effects of selenium in selenium-enriched malt at different levels on liver function and histopathological change which induced by DENA in male healthy rats were observed. One hundred and forty-seven healthy SD rats were randomly divided into three groups. Thirty rats in group I were fed with 0.1 mg selenium from selenium-enriched malt in per kilogram diet, designed as normal control; eighty-five rats in group II were fed with 0.1 mg selenium from selenium-enriched malt in per kilogram diet, designed as hepatocarcinogenesis control; thirty-two rats in group III were fed with 0.3 mg selenium from selenium-enriched malt in per kilogram diet, designed as selenium control. The rats in group II and III drinked the water with 100 mg L DENA. During the following days, body weight, the activity of AKP, ALT and the levels ALB of in serum, mortality and liver histopathological change of rat in three groups were monitored and recorded. After drinking the water with 100 mg L DENA for 14 weeks and drinking water without DENA were provide for another 4 weeks, after that, all of the rats were killed. Then the body weight, weight of liver and size of tumor were recorded, and the weight ratio of liver to body, mean weight and volume of tumor, inducing-tumor and restraining-tumor rate were calculated, and histopathlogical section (HE staining) of liver were inspected. The results showed that the content of ALB in plasma increased and activity of AKP and ALT in serum decreased when fed with 0.3mg/kg selenium; the mortality, the ratio of liver to body weight, mean weight of tumor, and the mean volume of liver tumor were lower than that of the positive control group; histopathological change of liver was alleviated compared with that of t... |
PDF Full Text Request