Study On Association Of HLA-DQB1 With Recurrent Spontaneous Abortion

Objective : To investigate the HLA-DQB1 genes polymorphism. and find the relationship between the recurrent spontaneous abortion and HLA-DQB1 . Methods : HLA-DQB1 genestypings are performed by polymerase chain reaction-restrictive fragment length polymorphism(PCR-RFLP) techmique in 61 cases of recurrent spontaneous abortion(of which 31 are ACA(+),30 are ACA(-), 22 have 2times abortions and 39 have 3times,28 are early abortions ,22 are late abortions and 11 are both early and late abortions) and 36 women without unnormal pregnancy history . Result :(1)In recurrent spontaneous abortion group ,the frequencies of DQB1*0201allele were increased highly ,but significant difference was not observed (P>0.05); the frequencies of 57 site with non Asp in RSA were much higher than that of control groups, and significant difference was observed ;(2) the frequencies of DQB1*0303 were found to be significantly higher in ACA(+) than in ACA( - ) (P<0.05) ; (3)the frequencies of DQB1*0201\0303 were significant difference among early abortion groups ,late abortion ones and both of them , through further comparing the frequencies of DQB1*0201 was significently higher in early abortion groups than that in late ones and the frequencies of DQB1*0303 were much higher in late ones than that in early ones .(4) There was no significant difference between the frequencies of 2 times abortion groups and 3 times ones. Conclusion : 57 site with non Asp may be sentisive gene to recurrent spontaneous abortions; DQB1*0201 may be related to the early abortions , DQB1*0303 may be related to the late abortions and abortions with ACA(+).