The Primary Research On The Formative Mechanism Of The Pericyst Of Hepatic Hydatid Cyst

Hydatid disease, or echinococcosis, is a parasitic condition of worldwide distribution. The disease has two kinds, infection with Echinococcus granulosus results in the formation of cysts and is called cystic hydatid disease or hydatid cyst; Infection with Echinococcus multilocularis results in the formation of parasitic tumors and is called alveolar hydatid disease. The disease is prevalent in Xinjiang, China. Because drug treatment of hydatid disease is still exploratory and has not yet been properly evaluated, surgical removal of the parasitic cyst remains the mainstay of treatment. During routine pericystectomy on hepatic hydatid disease, we found a previously un-describable fibrous membrane covering the exocyst, with a potential plane between the exocyst and the unknown fibrous membrane, which was called by we as "exo-adventitia" . It was easy to detach hydatid cyst along the plane with a lower blood loss.The aim of this paper was to study the formative mechanism of the exocyst and "exo-adventitia " of hepatic hydatid cyst and the potential anatomical plane between them. HE stain, immunohistochemistry and in situ hybridization were used to observe the histopathologic changes and the expression of hepatic fibrosis correlative factor: collagen IV, fibronectin(FN), laminin (LN), transforming growth factor- β 1 (TGF- β 1) and tumor necrosis factor- a (TNF- a ) in 40 exocysts and "exo-adventitia " of hepatic hydatid cysts and the surrounding hepatic parenchyma.Results showed that Pathologic changes of granulomalike were observed in the exocysts. A new fibrous membrane ( "exo-adventitia" ) in which there were many depressed vessels of Glisson capsules and hepatic veins was found between the exocyst and the hepatic parenchyma.A dividing line of staining of Collagen IV, FN and LN between exocyst and "exo-adventitia" was present. The positive rates of collagen IV, FN and LN were 85.0%, 87.5%, and 82.5% in the "exo-adventitia" , while they were 40. 0%(P<0. 01), 50. 0%(P<0. 01), and 37. 5% (P<0. 01) in the exocyst. Rate of positive cells of TGF- β 1mRNA and TNF- a...