Construction And Expression Of Recombinant Adenoviral Vector Carrying EBV Immediately Early Gene BZLF1

OBJECTIVE To construct recombinant adenoviral vector carrying EBV immediately early gene BZLFl by homologous recombination in bacteria and to detect its expression in vitro.METHODS EBV immediately early gene BZLFl were amplified by RT-PCR and were cloned to adenoviral shuttle plasmid pAdTrack-CMV. Then the resultant pAdTrack- CMV-BZ was cotransfected into BJ5183 bacteria with the plasmid pAdeasy-1. The adenoviral plasmid carrying EBV immediately early gene BZLFl was generated with homologous recombination in bacteria and the adenoviruses were produced in 293 cells. 293 cells was infected with adenoviruses and the expression of BZLFl was detected by RT-PCR and Western blotting in vitro. RESULTS BZLFl was expressed efficiently in 293 cell after infection. CONCLUSION The recombinant adenoviruses expressing BZLFl was constructed successfully and can be used further in gene therapy of EBV.