The Controlled Release Of RhBMP-2/type I Collagen In Dog's Allo-transplanted Trachea And It's Effect On Local ALP Activity

Tracheal transplantation is one of the important anticipated events in tracheal surgery, regarded as an ideal technique for reconstruction of tracheal defect after extensive resection. The research on tracheal transplantation has progressed slowly in the past years because of the subsequent severe stenosis and obliteration of the tracheal grafts. The major causes consisted of ischemia and immunological reaction to tracheal grafts, which result in cartilaginous necrosis, chondrolysis, resorption and connective tissue accumulating within the lumen. Our experiments have shown that local application of bone morphogenetic proteins (rhBMP-2) can induce cartilage regeneration and sustain the graft trachea. In this study we investigating the controlled release profile of rhBMP-2/type I collagen composer and it's affect to alkaline phosphatase(ALP).Objective: Aim to induce cartilage regeneration in tracheal grafts by utilizing the osteogenesis character of rhBMP-2, and ultimately aim to investigate the continous release time of rhBMP-2 from rhBMP-2/ type I collagen and time-affect relationship between rhBMP-2 release and ALPs activity.Methods: Canine tracheal transplantation models with muscle flaps surrounding the graft were created and rhBMP-2 was implanted into the canine...