Study Of Novel Fluorescent Oxygen Probe

Oxygen is the basic substance to maintain life and to keep the function of organs. Abnormality of the oxygen concentration and distribution in any life system will affect the normal physiological activities directly. Recently, attentions have been focused on researches of oxygen in biology, biomedicine fields and the pathogenesis involved oxygen. At present, oxygen detection mainly based on the fluorescence and phosphorescence quenching of probes, but there are some deficiencies of existing probes. It is necessary and exigent to develop novel fluorescent oxygen probe, which is water-soluble, long wavelength, well response, innoxious and non-invasive, so as to detect oxygen in vivo directly without distinct disturbance of the background.The thesis includes four parts: Part Ⅰ. ReviewThe analysis method and mechanism, quenching theory of oxygen on fluorescence and phosphorescence, fluorescent and phosphorescent oxygen probes and their application in biomedicine field have been elaborated summarized in this part. Part Ⅱ. Synthesis of novel water-soluble metalloporphyrins1. Meso-tetra(4-hydroxylphenyl) porphyrin(H2THPP) was synthesized by using the method of mixed solvents. The sodium meso-tetra(4-hydroxyl-3-sulfonate-phenyl)-porphyrin(T(4-HP)PS4Na4) was synthesized by sulfonating H2THPP under the proper condition through salting-out method. The overall yield was 68.5%.2. The optimal synthetic condition of palladium, platinum and ruthenium porphyrin was explored. Three water-soluble metalloporphyrins were synthesized at room temperature in a Britton-Robinson buffer solution. The composition of the metalloporphyrins was metal: porphyrin=1:1.Part Ⅲ. Study on the properties of three water-soluble metalloporphyrins1. The absorption spectrum, fluorescence spectrum, and the characteristics of fluorescence quenching by oxygen and reactive oxygen species have been studied systematically. The experimental results showed that, compare with porphyrin ligand, the formation of metalloporphyrin complex results in a modest blue shift of Soret absorption, decreasing of absorbance, with the Q band being reduced to single. Metalloporphyrins emitted distinct red fluorescence at room temperature (λex/λem and...