| IntroductionHeat shock proteins (HSPs) are a group of proteins that can be found in almost all kinds of the body cells. They are highly conservative in evolution, but play an important role in maintaining normal function of the cell. The HSPs in the cell may over-express under various stress situation in order to protect the normal cell function.The HSPs can be divided into tiny HSP, HSP10, HSP40, HSP60, HSP70, HSP90, HSP110, and HSP60, etc., accordingto the degree of homology and that of molecular weight. The HSP70 and HSP60 are highly conservative in evolution, and very important in HSP family.HSP70 has already been studied vastly. It may express abnormally in tissues of the tumor, by combining with the production of oncogene and the anti-oncogene. It may take part in controlling the cellular cycle of the tumor cell. It may also affect the multiplication, the apoptosis, the differentiation, the multi-drugs resistance, the tumor immunology, and the development of the tumor cells. But, we still know less now about the HSP60, especially on its role in oral disease and oral tumor.The oral squama cell cancer is a common oral tumor with a high malignancy and a bad prognosis. On the other hand, Leukoplakia is the most common precancerous change in oral mucous membrane. There is still no effective therapy now for the leukoplakia. About 16%-32% of the case in the leukoplakia may develop into cancer. Therefore, to elucidate the express of HPSs in the membrane of precancerous change (leukoplakia) and the squarma cell cancer may help us to make progress in early diagnosis, prognosis judgment and evaluation of the treatment.The purpose of the present study is to examine HSP60 express in the oral leukoplakia and the squarma cell cancer, and to find the relationship between the HSP60 express and the development of the leukoplakia. We also look for useful evidence to explain the role HSP60 plays in the development of the cancer. We believe these evidence may help us to make progress in the knowing the happening, the development, and the treatment of oral cancer.1. Materials and MethodsMaterials from 97 leukoplakia or cancer cases were divided into four group: light, moderate, sever and cancer group. The clinical diagnoses of all these sample cases were evaluated through the original methods of pathology under the optic microscopic with HE coloration. Immunohistochemical staining was used in clinical specimens of 79 cases of human oral leukoplakia and 18 cases of oral squamous carcinoma with monoclonal antibody against human HSP60.The data were treated according to double blind-control statistically. The cell was defined the masculine cell when we find brown color in its cytoplasm. We counted the masculine cells in 20 fields of visions in high times. All the cases weredivided into negative, light positive, moderate positive and strong positive group, according to percentage of masculine cells. The last two were the over-expression cases. The test data was analyzed with Statistica5.0.2. ResultsThe brown color was strained in the HSP60 positive cytoplasm. A smattering of cells was strained on cytomembrane. There was no straining in the nucleus in all cells. In the most cases the HSP60 expressed in the light and moderate dysplasia cases were in negative or light positive. The positive rate was partly in 30.00% and 50.00%, while in the sever dysplasia and squamous cell cancer cases the HSP60 express was always in moderate positive and sever positive.The positive rate of HSP60 in these two type cases was 68.42% and 88.90%. The over-expression rate of HSP60 was 55.6% and 72.22%. The positive staining rate of HSP60 in sever dysplasia and squamous cell cancer was significant higher than that in light and moderate dysplasia (P<0.01).There was no significant different between in severe dysplasia and squamous cell cancer (P>0.05). But the over-express rate between the dysplasia and squamous cell cancer wassignificant (P<0.05).3. Discussion and ConclusionHeat shock proteins (HSPs) are also called stress proteins. Secreted... |
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