| Gastric cancer continues to be one of the most common malignancies in china, which severely influence the public health. The mortality about 23.02% accounts for the leading cause of death among malignant tumors. The occurrence and development of gastric cancer is a complicated process involving multi-factors and multi-genes. During this course, the malfunction of proliferation of cells resulting from the inactivation, mutation or irregulation of prooncogenes, suppressive oncogenes and other molecular leads to malignant conversion. And angiogenesis play an important role in the advancement and metastasis of gastric carcinoma. So it is important to understand to get the information of the biology behavior and the prognosis that we look for the differential indix of occurence, advancement and metastasis of gastric carcinoma. No associated study at home and overseas that loss of PTEN influence the angiogenic inhibitors (endostatin) and promoters (VEGF) was reported. Therefore, we investigated the expression of PTEN, endostatin, VEGF and MVD of gastric carcinoma by immunohistochemical streptavidin-biotin peroxidase methods (SP) in 102 surgical resected specimens, and the relationship between them and occurrence, advancement, many clinicopathologic parameters and prognosis of gastric cancer were analyzed.Materials and Methods:Surgical specimens of 102 patients with histologically confirmed primary gastric adenocarcinoma including gastric tumor and paracancerous tissues (more than 5 cm away from the lesion) were obtained from Department of General Surgery, the No 2 Affiliated Hospital , Zhejiang University School of Medicine from February 1997 to October 1999. Each specimen was classified according to the Borrmann' s classification, China histological classification criteria and UICC TNM classification. Samples were fixed and embedded in paraffin as usual. Slides were used for HE staining and SP immunohistochemical staining, the latter was used to detect the expression of PTEN, VEGF, endostatin and MVD.Results:1. The PTEN expression was more intense in paracancerous normal tissues in comparison with in gastric carcinoma (100% vs 63. 7%, respectively, P <0. 01). The relationship between PTEN expression and invasion depth, differentiation, lymph node metastasis, distant metastasis, or vessel invasion of gastric cancer is closely (P<0.05), but the significant difference between the PTEN and Borrmann type , degree of the size , differentiation and Lauren type of gastric cancer were not found. Inactivation of PTEN contribute to the occurrence, development and metastasis of gastric carcinoma.2. The positive rate of endostatin expression is significantly higher in paracancerous tissue than that in gastric carcinoma tissues (62.7% vs 22.5%, respectively, P<0.01). The positive rate of endostatin in gastric cancer with Borrmann III and VI ( 29. 2% ) was not higher than that with Borrmann I and II ( 13.5%, P>0.05). The relationship between endostatin expression and invasion depth, differentiation, lymph node metastasis, distant metastasis, or Lauren type of gastric cancer is closely (p<0.05).3. The positive rates of VEGF in gastric carcinoma tissues were 67.6%, and significantly higher than that in the paracancerous tissues (34. 3 %, P<0. 01). The expression of VEGF was closely related to the invasion depth , lymph node metastases , distant metastases, histological type, vessel invasion, and Lauren type (P<0. 05) . The expression of VEGF had no significant difference between the different Borrmann type and degree of the size.4. The mean value of MVD in gastric carcinoma was 28.49 ± 9.16. The expression of MVD was closely related to the invasion depth, lymph node metastasis, distant metastases and vessel invasion (P<0. 05), but there was no significant correlation MVD expression with the size, Borrmann type, Lauren type and histological type.5. The mean value of MVD in endostatin negative group is 30. 18 ± 8. 57, and higher than that in endostatin positive group (22. 70 ± 8. 92, P<0. 01). The mean value of MVD in VEGF positive... |
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