| Objective To study the optimal time for bone marrow cells transplantation after myocardial infarction. Methods 36 Rabbits were divided into 6 groups(n=6), including immediate control group, immediate transplantation group, 2 weeks control group ,2 weeks transplantation group, 4 weeks control group, and 4 weeks transplantation group. The left ventricular free wall was frozen by liquid nitrogen. Culture medium (control groups)or bone marrow cells (transplantation groups) induced by 5-aza and labeled by Brdu were transplanted immediately(n=6),2 weeks(n=6),and 4 weeks(n=6) respectively after myocardial infarction. In forth week after transplantation, heart function, histologic studies, and immunohistochemical staining were performed. Results In immediate group and 4 weeks group, transplantation groups were better than control groups in EF, FS, dp/dt max,(p<0.05) but similar to control groups in LVDD and LVSD.(p>.05) The 2 weeks transplantation group was better than control group in EF, FS, dp/dt max, LVDD, and LVSD.(p<0.05 and p<0.01) Bone marrow cells labeled by 5-aza appeared in scar in 2 weeks and 4 weeks transplantation group, but did not appear in immediate transplantation group. Only in 2 weeks transplantation group, Bone marrow cells transplanted seemed to form cardiac tissue within infraction region. Conclusions The optimal time for bone marrow cells transplantation is at 2 weeks after myocardial infarction in rabbit model. |
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