The Expression Of BFGF With Bone Marrow Stromal Cells Injected After The MCAO For 1 Hour In Rat

ObjectiveTo investigate the expression of bFGF in rat with bone marrow stromal cells injected after the MCAO for 1 hour was induced and to study the protective effect of BMSCs against neuroal injury and the possible roles of alteration in the expression of bFGF following cerebral ischemia in rat.MethodsForty - five wistar rats were randomly assigned in3 groups: BMSCs - treated group,saline group ,nothing group , there were 15 rats in each group . Firstly, temporary ischemia of the middle cerebral artery occlusion (MCAO) for 1 hour was induced by the suture occlusion technique . Secondly, after BMSCs were proliferated, marked with BrdU and pre - diferentiated in vitro , the BrdU -marked BMSC were injected into rats in the experimental and control groups through vein systems, and then their distributions were observed in varied brain areas histologically. Finally, according to the methods above - mentioned to make MCAO model and vein injected into rats successfully,we measure these folllow-ing things, these rats were killed at 3. 7. 14 days after cerebral ischemia scores of neurological deficits could be used to assess the improvement of neurological function and at the same time HE staining immunohistochemistry staining for BrdU . immunohistochemistry of bFGF: 10% the water match chlorine aldehyde, 3. 5ml/kg belly carities inject the anaesthesia, then with 4% multitude gather formal dehyde to left vein. Fixed, packed, cut into slices, applied SABC method . The first antibody which is bFGF IgG of rat antibody multitude (brought from Wuhan Baster Company). The second antibody is a biotitude goat anti - rat.DAB to show the colour , normal regulations to dehydrate, transparent, sealing shices. Finally, We detect the expression of the bFGF after 3,7,14 days of cerebral ischemic reperfusion by microimage analysis system and irrnnunohistochem-istry.ResultsSignificant recovery of neurological function was found in rats treated with BMSCs . Comparing with the contrasted group, no striking difference, After induction BMSCs transplantation in MCAO + MSCs group neurological deficits alleviated significantly at 14days compared not only with the other groups at the same time points by with the same group at different time points, the amount of-BrdU Positive cells within damaged areas in auto tranaplantation groups were Significantly larger than that in the symmetrical brain area. Expression of bFGF protein ; no obvious difference between the saline group and nothing group ,the expression is no strinking difference at each temporal point after lacking of blood (P > 0. 05 ) , The expression of the BMSCs group at each temporal point increased strikingly (P < 0. 05 ).ConclusionsWe injected MSCs into the rats' vein soon after temporary ischemia of the middle cerebral artery, occlusion (MCAO) for 1 hour, Intravenous injected MSCs is simple and effective on improvement of nerological functional deficits after storke in rats . the effect result of this experiment shows: comparing the group (inject MSCs into vein)with the former two groups,the expression of each temporal point after lack of blood increased obviously (P <0.05) ,The increase of bFGF in the ischemic tissue play an important role on nerological recover, it protects nerve cell and nerological cell and promote new vessel to form etc , therefore it protects its brain.