The Study Of Protective Efficacy Of Combination Of Lung Protective Ventilation Strategies And Anisodamine In A Rat Model Of Acute Lung Injury

Objective To investigate the efficacy of combination of lung protective ventilation strategies and anisodamine in animal model of acute lung injury . Method 40 rats model of acute lung injury induced by oleic acid via injection of tail vein were randomly assigned into five group (8 rates each group ) after being anesthetized ; control group(group A); treatment with anisodamine (5mg/kg/h) ( group B); ventilation with large tidal volume (15ml/kg) ( group C) ; ventilation with small tidal volume (5ml/kg) ( group D) ; combination of ventilation with small tidal volume and anisodamine (group E) .The rats of C, D, E group were instrumented with tracheostomy and intubation to receive ventilation , and then to assay arterial blood gas and mPAP before treatment as well as post-treatment every hour for four hours .Eventually , all rats were killed . The lung were removed from the thoracic cavity , and preserved in formalin for histologic examination. Results Oxygenation index (PaO2/FiO2 , OI) in group A rats droped progressively , compared with pretreatment , which at 2h , 3h , 4h had significant difference (P<0. 05) ;0I in group B demonstrated the trend of decrease , which increased significantly at 3 and 4 time point; OI in group D and E rised constantly , which among different time points almost had obvious discrepancy ; OI in group C wentup apparently at former tow time point , which higher than that of any other groups , but drop marvelously at later tow time points, as compared with other groups , is lower ; OI in group E were the highest at 3 and 4 time point ; Between group A and D as well as D and E , without the statically difference . MPAP in group A showed the increasing tendency, which at respective time points were obviously higher than that of pretreatment; MPAP in group B, D , E , declined persistently , which at 2, 3, 4, time points were higher than that of group A; MPAP in group C decreased more sharply at previous tow hours , and increased more obviously at the third and the fourth time points than that of any other groups (P<0.05) .Ultimately, the lung injury score in group C was the highest among five groups (P<0. 05), which in group E was the lowest ; The lung injury score in group B and D were lower , compared wih group A. Conclusion combination of lung protective ventilation and anisodamine have cooperative effect on animal model of acute lung injury.