The Study Of Interaction Between Rare Earth Ion-Drug Complex And Biomolecule And Its Application

Human Serum Alubmin is the most of abundance protein in blood. The study of it is not only offers the material basis for the law of lifecycle, but also offers the theoretical basis and resolvent for the interpretation of many morbid mechanisms. There is a connection between the content of HSA in urine and some diseases, so the determination of HSA is very important in clinical diagnosis.Lysozyme is a 14.6-kDa single chain protein of amino acids. It is present in several body fluids, including serum, urine and tears. The determination of lysozyme is very useful in clinical diagnosis, toxicological experiments and investigation of the influence of environmental pollution on human health for there is a connection between the content of lysozyme in body fluids and some diseases. For example, lysozyme is found at a much higher concentration in serum and urine of some patient than of healthy subject, especially those who suffered for leucocythemia and nephropathy or for many heavy metal ions including cadmium and mercury. So it is of special value to determination lysozyme in clinical diagnosis.The study of nucleic acid is of great importance in biochemistry, biotechnology, and pharmacodynamic and other areas. Today, the study of nucleic acid using fluorescence probe has attracted more and more attention.In this paper, using molecule spectroscopy (fluorescence and Uv-Vis spectroscopy), we study the interaction between drug-metal ion complex and biomolecule such as HSA, lysozyme and DNA and its application. There are three chapters.First, using Eu3+-tetracyclines complex as fluorescence probe, study the interaction between the complex and HSA and its application. Tetracyclines contained 3 -diketonate configuration, they are ideal ligands for Eu3+ ion and it is possible to sensitize thefluorescence intensity of Eu3+ ion via intramolecular energy transfer. Adding HSA to system, the characteristic fluorescence intensity of Eu3+ could be enhanced greatly, and the enhanced fluorescence intensity is in proportion to the concentration of HSA. According to this, we constructed a new method for the determination of HSA.Second, using Eu3+-tetracyclines complex as fluorecence probe, study the interaction between the complex and lysozyme and its application, this tetracyclines including doxycycline, metacycline and tetracycline. Applying fluorescence quenching method and energy transfer theory, we have studied the quenching mechanism of protein and drugs. The binding constants were obtained in the absence of metal ions. According to the thermodynamic parameters, the main sorts of binding force can be known. After addition lysozyme to Eu3+-drug system, the characteristic fluorescence intensity of Eu + could be enhanced greatly, and the enhanced fluorescence intensity is in proportion to the concentration of lysozyme. According to this, we constructed a new method for the determination of lysozyme. The developed method was practical, simple, sensitive and relative free from interference coexisting substances and had been successfully applied to the determination of lysozyme in serum and urine samples.Third, there is energy transfer between oflaxin and Tb3+ and the complex can emit the characteristic fluorescence of Tb3+, so the complex can be used to determine DNA as a kind of fluorescent probe. In addition, the hydrophobic environment can dramatically increase the fluorescent intensity of complex. In this part, we studied the fluorescence increased effect of nucleic acid on the complex system, explained the interaction mechanism. We also study the interaction between Al-Daunoblastina and DNA and its application.