The Study Of Mast Cells And Macrophages In Recurrent Aphthous Ulcers

Objection:We aimed to investigate the expression of mast cells (MCs)and macrophages( MΦ)in recurrent aphthous ulcers (RAU) and study the relationship between the cells and disease., which helped to study pathogenesis and select therapy.Methods:Biopsy specimens of RAU were obtained from fifteen patients. For comparisions, biopsies of induced oral TU from twenty patients were used, in addition to twenty control biopsies from the oral mucosa of healthy volunteers with a negative history of RAU(age range 28-45 years).The expression and localization of MC and Mφwere examined in biopsies specimens by immunohistochemistry. Positive cells of the sections were counted by Image Analysis System. Five visual fields(10X10)were selected at random. Differrences among groups were compared by SPSS10.0 statistics software using Kruskal-Wallis test. P<0.05 was selected as significant standard. And the expression and localization of MC were examined in biopsies specimens by electron microscopy.Results: Positive position of tryptase or CD68 located on cell plasm with brown in MC or Mφ..Monoclonal antibodies specific for tryptase and Monoclonal antibodies specific for CD68 were used to identify and distribution of MCs and Mφin an immunohistochemical study. In RAU lesions, numerous tryptase-positive MCs were found in the sub-epithelial lamina propria, and MC numbers in the epithelium were present only in some RAU lesions. MCs counts in RAU-infllammatory infiltrates-connective tissue were significantly greater than those seen in TU- infllammatory infiltrates and control(P<0.05)。MC activation /degranulation, as judged by diffuse extrcellular tryptase staining, was a common feature within RAU-infllammatory infiltrates-connective tissue interfaces, which were associated with mucosa tissue disruption. CD68-positive macrophages were found in RAU-infllammatory infiltrates-connective tissue were significantly greater than those seen in TU- infllammatory infiltrates and control(P<0.01). By electron microscope, MCs in RAU group contained granules with particulate substructure. In RAU lesions, there were closed relations between MCs and fibroblasts, lymphacytes; Mφwas actived, there were closed relations between Mφand lymphacytes.Conclutions: The expression of MC and Mφ were examined was higher in RAU than TU and normal control. There were closed relations between MCs and lymphacytes. And there were closed relations between Mφand lymphacytes, and show signs of activation/degranulation gestion active involvement of these cells in RAU pathogenesis, which helped to study select therapy.