Role Of Hepatocyte Growth Factor And Lepein In Diabetic Retinopathy

Objective: To explore the role of hepatocyte growth factor and leptin in the progression of diabetic retinopathy, and to provide a new method to diagnose early and therapy effectively DR.Methods: Seventy Wistar rats were divided randomly into two groups. There were thirty in normal control group(NC) and forty in diabetes mellitus group(DM). The subjects in diabetes mellitus group were injected intraperitoneally with low dose streptozotocin(STZ, 30mg/kg) after high calorie diet given for two months, while the ones in normal control group were injected intraperitoneally with the similar dose of citric acid buffer. Before injection, one month, two months, three months and six months after injection, there were six rats in both groups respectively killed. The blood samples were obtained to measure the levels of HGF, leptin, insulin, cholesterol, triglyceride, et al. The retina samples were used to analyse morphologically. We compare these data between the two groups and among the different stages with factorial designed analysis of variance to reveal the role of HGF and leptin in the pathogenesy of DR.Results: 1.There was a difference in the level of fast blood glucose between NC and DM after high calorie diet given for two months(P<0.01). The difference was significant after injected intraperitoneally with STZ and the level of FBG was increasing with the progression of DM, then at the level of 16.5-16.8mmol/L stably. In addition, there were a significant difference in the levels of triglyceride and cholesterol between the two groups at every stages(P<0.01). These changes are similar to type 2 diabetes mellitus.2.Compared with NC, there were significant differences in the level of INS and insulin resistance index after high calorie diet given for two months (P<0.01). The differences between the two groups had existed for the whole course of the experiment. The phenomena are also similar to type 2 diabetes mellitus.3.The level of HGF of DM was much higher than NC. It increased gradually after injected with STZ and was at the highest level at two months. Then it decreased gradually. 4. The level of leptin was much higher than NC after high calorie diet given for two months(P<0.01), but decreased after injected with STZ, then it increased continually during the whole experiment.Conclusion: 1.High blood insulin and insulin resistance were made in the rats after high calorie diet given for two months. Then the rats were given low dose STZ to destory some beta cells. All of these were similar to the process of type 2 diabetes mellitus.2.In our experiment, the serum levels of HGF and leptin were higher than normal control. Our results indicate that HGF and leptin might be involved in the pathogenesis of DR.