| Objective: Rouganxiaozheng granule(which is composed of fresh membranous milkvetch root,parched largehead atractylodes rhizome,aromatic turmeric root-tuber,nutgrass galingale rhizome, green tangerine peel,white peony root,common burreed rhizome,zedoary,dan-shen root, eupolyphaga seu steleophaga,et al) possesses the function of eliminating blood stasis and lumps, relieving the depressed liver, soothing the liver, regulating the circulation of Qi,and repleniching Qi to invigovate the spleen, which was the effective prescription screened by professor YANG Muxiang through many years clinical practice and has the notable curative effect on treating hepatic cirrhosis.Further for probing the functional mechanism of Rouganxiaozheng granule, reproduced the rats models of cirrhosis adopting complex factors,then supplied with drug treatment ,to study the functional mechanism of protecting hepatic function through observing the effect of Rouganxiaozheng granule on aspartate aminotransferase(AST), alanine aminotransferase (ALT), Total protein(TP),and albumin(Alb); to study the functional mechanism of inhibiting immune injury through observing the effect of Rouganxiaozheng granule on tumor necrosis factorα(TNF-α), interleukin-1β(IL-1β), interleukin 6(IL-6),and transforming growth factorβ1(TGF-β1); to observe the effect of Rouganxiaozheng granule on pathomorphology of live tissue through HE and Masson staining.Methods: 60 Wistar rats,among which 48 rats were randomly selected for making model group and the rest were as normal group after normal feeding for 1 week。At first time,the making model group were subcutaneous injected by solution of 40% CCl4-arachisoil,0.5 ml per 100 gram,afterward,0.3 ml per 100 gram,two times each week,total 9 weeks. The rats were fed by mix animal feeds of 20% lard,0.5% cholesterol,and 79.5% maize flour for first 2 weeks,then were fed by mix animal feeds 0.5% cholesterol,and 99.5% maize flour for the later 7 weeks. Ethyl alcohol was as drinks for rats drinking during whole period of making model.The mormal group were fed by common animal feeds and clean water.On the end of 9th week , success of making model was affirmed.40 Wistar cirrhotic rats were randomly divided into 4 groups,10 rats per group,female and male were half.①Normal control group(in brief normal group):1 ml per 100 gram,one time each day,watering-stomach with saline.②Model control group(in brief model group): 1 ml per 100 gram,one time each day,watering-stomach with saline.③High-dose Rouganxiaozheng granule group(in brief high-dose group): 0.32 gram per 100 gram,one time each day,watering-stomach with Rouganxiaozheng granule suspension.④Low-dose Rouganxiaozheng granule group(in brief Low-dose group): 0.16 gram per 100 gram, one time each day,watering-stomach with Rouganxiaozheng granule suspension.⑤Positive control group(in brief control group): 0.13 gram per 100 gram,one time each day, watering-stomach with suspension of complex Biejiaruangan tablet. From the second day after the end of making models, all rats were continuous watering with saline or medicine for 4 weeks,and were anesthetized with 10% chloral hydrate (0.2ml/kg)after the last time medication and fasted for 12 hr.Afterward ,incised in abdominal middle, obtained blood from abdominal aorta, blood centrifuged with hypothermia,and serum separated,then to detemined the contents of ALT,AST,TP,Alb in serum,and detemined the contents of IL-1β,IL-6,TNF-αin serum by method of radiation-immunization.Meanwhile,took out the animal liver and spleen,absorbed the fluid of surface,weighed the wet weight,and calculated the index number of liver and spleen .Taken hepatic tissue about 1cm×1cm×1cm size from the largest foliage of liver, which were fixed with 4% citromint,embedded with paraffin commonly,and maken slice,to observe the expression of TGF-β1 in hepatocyte with method of immunohistochemistry,as well as observe the changes of pathomorphology of live tissue through HE and Masson staining. Results1 The effect of Rouganxiaozheng gran... |
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