| Background and aimThe breast cancer is the majority of malignant tumor. Breast cancer is becoming the highest incidence of cancer in women , and its harms to adult woman and society are increasingly predominant .Recently, it was identified that there was a close relationship between E-cad, -cat, c-erbB-2 and the occurrence , progression , biological character in most of cancers. E-cadherin is a 120kd transmembrane glycoprotein .It consists of three segments: extracelluar region, transmembrane region and intercellular region. Its intercellular region can interact with -catenin, the latter mediate the connection to the actin cytoskeleton via a- catenin. The extracelluar domains form zipper-like complexes predominantly with E-cadherin molecules from adjacent cells at point of intercellular contact .E-cadherin/catenin complex play a key role in maintain of normal epithelial form, among which, E-cadherin have a crucial role in maintain of integrity of normal epithelial cell, preventing tumor cells vicariance. abscission .Recently lots of pathological studies have showed that the loss or downregulate of expression of E-cadherin is a common phenomenon among malignant tumor ,always with loss of polarity of de -differentiation ,reinforcement of invasion ability, increasing of rate of lymph nodes metastasis. -catenin is a special cytoplasmic protein ,it can form E-cadherin/catenin complex by combination with E-cadherin , a -catenin and -catenin. It extensively exists in all types of epithelial cells and other types of parenchyma cell, mediating homotype cell-cell adjacentjunction . E-cadherin/catenin complex can suppress invasion and metastasis of tumor. Decreasing of expression of E-cadherin coincide with decreasing of E-cadherin/ catenin complex, it make cells impossible to maintain primary form and adjacently junction between cell and cell,subsequently, breaking away from tumor ,facilitating metastasis of tumor . C-erbB-2 is also called HER2, belonging to the family of EGFR. This gene is located at chromosome 17 qll-q12 and encodes a 185-kD transmembrane glycoprotein (p185 ), having intracellular tyrosine kinase activity and an extracellular domain that is very similar to the EGF-binding domain of the EGF receptor(EGER). when EGFR is integrated with EGF, Morphologically we can also find that the connection between cells become sloose, the initiative of cells increases and neoplasm cells become more aggressive. C-erbB-2 may suppress the function of E-cadherin/catenin complex The relationship of E-cad, -cat and c-erbB-2 in tumors cannot be found domestically.In our study, we detected the expression of E-cad , -cat, C-erbB-2 in breast cancer by immunohistochemistry and probed their relationship with biological character and clinical pathology in breast cancer, Materials and methodsWe collected 116 breast cancer samples which were resected by surgery and confirmed by pathology between 1993 and 2002. According to WHO grading standard of pathology, 36 cases were G1, 34 cases were G2 and 46 cases were G3; According to TNM standard of clinical staging ,48 cases belonged to I - II group, 68 to III-IVgroup. 10 benign breast lesion samples were selected as controlling group. In our study, we detected the expression of E-cad, -cat, C-erbB-2 in breast cancer by immunohistochemistry S-P method and analyzed their relationship. Results1. All 10 benign breast lesion samples were positive expression with E-cad and -cat,but no one was positive expression with C-erbB-2.2. The positive expression rate of E-cat was 42.2% in 116 cases of breast cancer.The positive expression rates of E-cat were 80.6% , 38.2%, 15.2% in GK G2, G3 of breast cancer and were 68.7%, 23.5% in T I-II, TIII-IV tumors. These expressions were related to the pathological grades, clinical stage (P<0.0 1 ; P<0.0 1 ).3. The positive expression rate of P -cat was 37.9% in 116 cases of breast cancer .The positive expression rates of P -cat were 66.7% , 44.1%, 10.9% in G1, G2, G3 of breast cancer, and were 64.6% , 19.1%, in TI-II, TIII-I... |
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