Experimental Study Of Chondrocytes Blended With Alginate And Insulin-like Growth Factor-I To Rebuild Cartilage In Vitro

Objective: To search an effective means to rebuild articular cartilage; Methods: Self-made three-dimensional scaffolds seeded with chondrocytes are cultivated in DMEM substrate for 4 weeks, IGF-I added in the experimental group; Results: Cartilage-like substances occurred in the second week and developed gradually. At the end of last week, mature cartilage are harvested. Conclusion: Chondrocytes are biocompatible to alginic acid calcium , and retain their original characteristic and the ability to form cartilage. IGF-I can promote chondrocyte's differentiation and proliferation, and maintain their original phenotypes.