| Gene transcription regulated orderly is the premise of the cells to main normal function. There are the intimate correlations between transcription regulation and the core histone modified by acetylation or deacetylation which is the function of a couple of adverse proteases——histone acetylases(HAT) and histone deacetylases(HDAC). Those form transcrition regulation compounds with each corrlate proteins which are Coactior(CoA) and Corepressor(CoR). If CoR bind with gene abnormally and HDAC act with histone continuously, gene transcription will be inhibited. Many tumors' occurrence and develop have the directly relations with transcription inhibition. Recently many compounds are found with the abilities to inhibit HDAC. The histone deacetylase inhibitiors(HDACI) have the antitumor abilities were reported in many studies, which include inducing tumor cell differentiation, blocking cell cycle and apoptosis. Those not only clarify HDACI antitumors molecule mechanism, but also explan the tumor's pathogenesis. Nevertheless the reports are less about HDACI to affect immunoloregulation. The previous investigations confirmed costimulatory molecules and adhesion molecules expression is down-regulation even absence in leukemia cells. Whether HDACI can inverse the situation or not? The study will be advangeous to clear the meachanism about down-regulation of tumor immune molecules and explore therapy pathway in molecule level. Moreover the study can offer the evidences for HDACI which apply to tumor therapy.The experiment select five cell strains as the study objects which include containing translation(15,17) human acute promyelocytic leukemia cell strain(NB4), containing translation(8,21) human acute myelocytic leukemia cell strain(Kasumi-1), no containing translation(15,17) human acute promyelocytic leukemia cell strain(HL-60), human acute monocytic leukemia cell strain(U937), human acute lymphoblastic leukemia cell strain(Jurkat). The cells were treated with sodium butyrate(SB) at 0, 0.1,0.5,1.0,5.0mM concentrations and in different time resoectively. Cell viability examined by both trypan blue dye exclusion and MTT reduction method, applied dual- or mono- agent analysis of variance, selected cell survival rate >85% and obvious gromth suppression groups concentration and time point which occur pre-, mid- and post- gromth suppression to use subsequence experiments. The expression of CD86,CD80 and CD54 were examined on the surfaces of five cell lines which were treated or not with SB by Flow cytometric analysis and data are analysed with ELETE software. Up-ragulation of CD86 ,CD80 and CD54 were detected at various levels on the cells treated by SB and with time-dosage-effect dependence. The bast effect is in the group at 0.5mM concentration in 48 hour point. But the levels of up-ragulation are obvious heterogeneity. The positive cell rates in NB4,HL-60,Kasumi-1 and U937 cells are increased more which come from acute myelocytic leukemia, however those are increased less in Jurkat which come form acute lymphoblastic leukemia ,statistics comparision is no difference with the control. Up-regulation of CD80 is with statistics significance in NB4 and Jurkat. In terms of CD54, a significant increase in its expression was examined in all five cells and statistics comparision is different with the control. NB4 cells treated with SB have an enhanced capacity to stimulate lymphocytes proliferation compared with untreated NB4 measured by allogeneic mixed lymphocytes reaction. This result support that costimulatory and adhesion molecules are functional which are up-regulated by SB. Nucleoprotein extract from cell treated with or without SB is tested to determined the effort on the changes of p65 and p50 which are main subunits of transcription factor NF-κB. To assess whether CD86 expression changes correlates with changes in nuclear NF-κB, the result reveals the increases of p65 and p50 are remarkable coherence with the changes of CD86. It is the evidence that NF-κB is one of the importa... |
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