| Objective To investigate the effects of electroacupuncture at Yongquan and Lidui acupoints on the reacton of lipid peroxidation. the activities of superoxide dismutase (SOD) and adenosine triphosphatase (ATPase) in rabbits' brain tissues during acute period of craniocerebral injury (CCI), and to explore the neuroprotective mechanism and the significance of electroacupuncture treatment during acute period of CCI.Methods 48 rabbits were randomly divided into group A (control group), group B (sham-operated group), group C (injured group) and group D (electroacupuncture treated group). Group C and D were further divided into subgroups of 3hrs, 6hrs and 24hrs. Referring to Feeney's method, the model of CCI was performed in group C and D by free dropping weight. The animals in group C were given electroacupuncture stimulation at non-acupoint areas of both hind limbs and the animals in group D were given electroacupuncture treatment at Yongquan and Lidui acupoints at 3hrs and 12hrs after CCI respectively. The scalps of animals in group B were incised and skull exposed, but CCI were not performed. Then the contents of brain tissue water, malonyl diadehyde (MDA) and the activities of Na+-K+-adenosine triphosphatase (Na+-K+-ATPase), Ca2+-Mg2+-adenosine triphosphatase (Ca2+-Mg2+-ATPase), CuZn-superoxide dismutase (CuZn-SOD) and mitochondrial Mn-superoxide dismutase (Mn-SOD) were measured.Results Results (1) Compared with group A and B, a significant decrease of consciousness score was observed in all animals that were subjected to free dropping weight impact (P<0.01); The consciousness scores in the animals of group D were higher than that of group C except 1h after CCI, but the changes were not significant; Histological examination revealed cerebral vascular hyperemia and hemorrhage in all animals subjected to CCI. (2) Compare between group A and B, there were no significant difference in the contents of brain tissue water, MDA and the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase, CuZn-SOD as well as mitochondrial Mn-SOD (P> 0.05). (3) Compared with group A and B. the contents of brain water and MDA were significantly increased at 3hrs after CCI (P<0.05), and kept on ascending with timeextended. But the activities of Na+-K+-ATPase. Ca2+-Mg2+-ATPase, CuZn-SOD and mitochondrial Mn-SOD were remarkably decreased after CCI and kept on descending with tune prolonged. (4) Compared with group C, the changes of the above indexes measured in group D were slighter. (5)correlation analysis revealed that there were a positive correlation between the contents of brain tissue water and MDA (r=0.843, P< 0.01), a negative correlation between the contents of brain tissue water and the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase. CuZn-SOD and mitochondrial Mn-SOD (the values of r were -0.847, -0.844, -0.799. -0.803 respectively, P<0.01 ), and a negative correlation between the contents of MDA and the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase, CuZn-SOD as well as mitochondrial Mn-SOD (the values of r were -0.854, -0.865, -0.909, -0.889 respectively,P<0.01).Conclusion (1) The free radical reaction in rabbit brain tissues was enhanced and the activities of ATPase and SOD were inhibited after CCI, which contributed to the development and progress of brain edema. (2) Electroacupuncture treatment during acute period of CCI in rabbits could alleviate free radical reaction and brain edema by improving the cleaning ability of free radicals and the activities of ATPase. |
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