The Study On The Anti-Epilepsy Constitutes Of S.chamaejasme

It has been shown that S.chamaejasme L has strong anti-cancer and antibacterial effects. It has also been reported that its water soluble contents, alcohol soluble contents, and volatile oil exhibit super-satisfied result in the treatment of anti-malignant tumor. At the same time, it was also used in the treatment of tuberculosis, skin disease, gynecology disease and beef tapeworm disease by the mixture. Recent research showed that it held the anti-Epilepsy activity. In order to investigate the material foundation of its anti-Epilepsy activity, the methods of the natural pharmaceutical chemistry and pharmacology were used to search for anti-Epilepsy effective part or components of S.chamaejasme L. The main research was aimed to identify the variety of the traditional Chinese medicine, to produce the different solvent extractions, to carry out the pharmacological experiment of anti-Epilepsy, to accumulate and purify the total flavonoid compositions and the total coumarin compositions of the two effective parts as soon as their own pharmacological experiments of anti-Epilepsy each other, to measure the total flavonoid compositions derived from the two effective parts (the ether extraction and the acetone extraction), to separate and identify the components from the ether extraction.To identify the variety of the traditional Chinese medicine: S.chamaejasme L is the variety in this topic through the characteristic identification, transverse section, medicine powder microidentification, specific qualification tests and TLC.To determine the anti-Epilepsy effective part: Firstly, based on the long-term research on the epilepsy using traditional Chinese medicine by professor Liu YuXi, the first hospital of Shanxi medical university, it had been found out that the cruel extraction of S.chamaejasme L exhibits some anti-Epilepsy activity in rats (ip) by 248mg/kg. So each of the group was administered the medicine (ip), the dosage of which was equel to the cruel extraction of S.chamaejasme L. According to the anti-Epilepsy effect of each extraction in rats, the ether extraction and the acetone extraction were determined to be the effective parts, which including control group (saline), based on the Maximal electroshork seizure test (MES) and Metrozol Seizure test (MET). But the ether extraction showed comparative toxicity as well as the anti-Epilepsy activity. The acetone extraction exhibits satisfactory anti-Epilepsy effect; Secondly, the total flavonoid compositions and the total coumarin compositions were gained from the two effective parts by separation and accumulation. According to the pharmacological experiment of anti-Epilepsy, it can be concluded that the mutual interaction may exists between the total flavonoid compositions and the total coumarin compositions. Through the measurement of the total flavonoid compositions derived from the two effective parts, the quantity of the ether extraction and the acetone extraction was 8.51%, 5.07%. Combined with the pharmacological experiment result, it can be concluded that the relationship may exist between its strong anti-Epilepsy effect and its high quantity of total flavonoid compositions in the ether extraction. But the research of its toxic components has to be further studied. To investigate its anti-Epilepsy and toxic components, further separation of chemical components from the ether extraction was carried out on the polyamide column chromatography and the silica gel column chromatography. As a result, three flavonoids were isolated from the ether extraction, which were identified as Chamaechromone, neochamaejasmin A and (+)-chamaejasmemin by means of physical, chemical methods and spectrum.