Pilot Study Of The Preparation Of Tissues Engineering Scaffold Materials By Supercritical CO₂ Fluid Technique

Because of the incomparable merits (nontoxicity, non-remainder, fast transfer mass) of supercritical carbon dioxide fluid technique (SC-CO2) , it was used to developed a series of novel biodegradable tussue engineering scaffold materials in this research. The relation between the morphologies of the materials and the treating parameters were first studied in this work. The glass transition temperature (Tg) and the cell toxicity of these materials before and after treated by SC-CO2 were tested by DSC and MTT-assay respectively. A series novel chitosan/PLA. hyaluronate/PLA. bFGF/PLA composites tri-dimensional porous scaffold materials were prepared under SC-CO2 conditions, with our basis study on how SC-CO2's swelling parameters will influence the morphologies of the material prepared by SC-CO2 technique. The results showed that the morphologies of these materials are changed correspondingly to the change of the reaction conditions. Compared with the original materials, the Tg of the treated materials was increased a little and the cell toxicity of the treated materials was reduced. Because of the functional ingredients such as chitosan, hyaluronate and bFGF(basic fabric growth factor) were compounded into the PLA, the saffold materials were improve a great in the aspect of mechanics character and the biocompatibility. All these results prove that SC-CO2, which can be used to prepare porous biodegradable scaffold materials with excellent tri-dimensional porous structure, high porosity, good mechanics intension, well cellcompatiblity and fine tissuecompatiblity without the use organic solvent, is a promising method in preparing tissue engineering scaffold materials.