| Objective: The globe rupture is especially complicated, which is very bad after treatment. This research use air gun bullet to make wound on habbit eyeball, then observe the early clinical manifestation , the pathology changes, and the injury mechanism of photoreceptors cell of the eyeball , supply experimental basis for clinical treatment.Methods: 48 white New Zealand rabbites were Selected ,of which the anterior sequecment eyes and fundus checks are nomal .The weight is 2.5kg to 3. Okg . Then with random figure form method , the rabbits were randomly divided into 6 groups , 1h,3h,6h, 12h, 24h, wound groups and control groups , each had 8 rabbits respectively. Anaesthetize the rabbits, the eye were shoot from the back costa of the rabbit's ear root and the middle line 1mm inside corneoscleral limbus with an air gun , the bullet projected out from the nose root. 1h, 3h, 6h, 12h, 24h. After the shooting electroretinogram was taken. Then execute the experimental rabbits , and enucleate the eyeball immediately . Cut off the cornea , fix the eyeball for 24 hours with 4% polyformaldehyde phosphate buffer ,then dehydrate it with alcohol gradually .Make sure the xylene is transparent , and embed it with paraffin for backup .Slice the well-embed eyeball specimen into 8 pieces, 4 m thickness in succession , and paralleled with arrowy axes of optic nerve and retina with it as plane. Carry through HE stain , the P53, bcl-2, cell originallocation apoptosis examines respectively to each two pieces . the HE dyed tissue slices were observed under super times microscopes (AUT-2000 TYPE super times microscopes diagnosis system ).Methods of light microscopes observation: Disposal TUNEL method cell apoptpsis ,P53 and bcl-2 albumen masculine expressing image by high-definition color pathology image analyse system (HPIAS-1000) (take four visual fileds , each visual field area is 0.2mm*0.2mm). And count the brown-yellow masculine cells in the visual area .Analyse the P53, the expression of bcl-2 and the apoptosis of cell quantitatively , all the original data were analysed with SPSS10. 0 statistics software ,proceeding variable convert and related analysis .Results1, clinical manifestationAfter being wounded ,the rabbit' s wound eye conjunctiva immediately manifested congestion, edema, and aqueous overflowed from the cornea, then the iris and lens emerged, 15s after injury to 1 miniute , intraocular hemorrhage eyeball internal bleeding overflowed the wound .2 electrooculography resultsThe normal electroretinogram ERG- b wave flaps as 103.5+16841 mv, the experiment group 1 h, 3 hs, 6 hs, 12 hs, 24 hs, the ERG- b wave of all disappeared, and manifested as a put-out type.3 pathologythe HE stain Observed by light microscopes and extremely high times microscopes : epichoroidal space bleed could be seen in all injured eyes, numerous red blood cells were observed in vitreous ,the retina detached , episclera the sclera bleeding was occassionally seen in thin layers, the sclera seperated . ectoretina the layers of the photoreceptor cells of the retina chip between 1 and 3 hours , the other tissue had no change, the inner nuclear layer and outer nuclear layers of the retinachanged obviously, ganglion cell nuclear shrunk alittle ,other changes like these obviously appeared between 6-12 hours, and while 24 hours the retina became thin and its structure is disordered .4 the immnohistochemistry and cell apoptosisThe bcl-2protein had no obvious expression in the control group and each experimental group , 3 hours latter , a lower positive expression of p53 were observed in the inner and outer layer of the nuclear and ganglion cells , it increased obviously between 6-12 hours, and decreased slightly after 24 hours .The apoptosis had lower expression in ganglion cells and the inner and outer nuclear layer 6 hours latter , an obvious increasing of dye positive cells was obsered between 12 and 24 hours. In the experiment :when checked by One-way ANOVA, there was no significant difference between bcl-2 group and each experimenta... |
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