Clone Culture Of Hybridoma Cells And Purification Of Its Secreting McAb

Objective: To purify monoclonal antibodyN-35.Methods:Firstly,to subclone hybridoma cell P-35 secreting monoclonal antibody N-35; then we make affinity chromatography column with proteinA-Sepharose CL-4B to purify monoclonal antibody N-35. Results: Purified monoclonal antibody N-35 is electrophoresised and can show single band and keep nicer immunocompetence,Conclusion: Limiting dilution used in subcloning cells isvery effective, Affinity chromatography through protein A-Sepharose CL-4B can purify monoclonal antibody effectively.