The Experimental Research On The Effects Of PhVEGF Gene Transfer On Vascular Smooth Cell (VSMCs)proliferation And Apoptosis In Mini-swine Vein Grafts

Objectives:To investigate the effect of ad-hVEGF165gene transfer on vascular smooth muscle cell proliferation and apoptosis after autogenous vein grafts.Methods:A mini-swine model of autogenous vein graft was established by transplanting the left external jugular vein into left common carotid artery infourty mini-swines. Immunohistochemical technique and terminal transferase DUTP-biotin nick-end labeling (TUNEL) were used to detect the expression and distribution of proliferation cell nuclear antigen (PCNA) and apoptosis of vascular smooth muscle cell(VSMCs)transferred by VEGF165 in vein grafts at different time.Result: The expression of PCNA and TUNEL of VSMCs in Ad-hVEGF165 and none-VEGF165 groups were markedly increased compared to control group ;(1)Ad-hVEGF165 groups induced significant decreased expression of PCNA in the media smooth muscle cells compared to none-VEGF165 groups in every graphs. On day 7, 14 and 21, the positive rates of PCNA were 39.6 + 5.4 VS 15.2 ?.6 (P<0.05), 34.7 + 6.6 VS 13. 6?.0 (P<0. 05) and 18. 7 ?.0 VS 9.6 + 1.3 (P<0.05) respectively. (2) Although Ad-hVEGF165 groups could not induce much more significant apoptosis in the media smooth muscle cells, the ratio of positive rates of PCNA to TUNEL were lower than that of none-VEGF165 groups, On day 3, 7 and 14, the ratio of2001positive rates of PCNA to TUNEL was 0.908 + 0.3 VS1.852 + 0.8 (P<0. 05), 0.666 + 0. 1 VS2. 630?1. 5 (P<0. 05) , 0. 788 + 0.3 VS 1.460?. 9 (P<0. 05) respectively .Conclusions: Local VEGF165 gene transfer could significantly restrain the VSMCs proliferation and phenotype changes and induce the apoptosis of VSMCs after autogenous vein grafts in rain-swine model, which can inhibit the intimal hyperplasia and prevent restenosis.