| Objective:1. Using the method of stereology to reveal quantitatively and systematically the characteristics and changing rules of the three-dimensional structure of the carcinomatous cell and its ultrastructure in the lung. To explore the value of the stereologic parameters of the lung carcinomatous nucleus on the diagnosing, sub-typing and grading of lung carcinomas.To evaluate the significance of the change in the lung carcinomatous cellular ultrastructure.2. To observe, measure and analyse the sructural characteristics of the intracellular microcyst in the lung adenocarcinoma. To study the process and mechamisms of its formation and evolution.Methods:A total of 78 lung carcinomatous specimens and normal lung mucosas from the lung surgical resections were collected. They were grouped into the squamous cell carcinoma group(a), adenocarcinoma group(b), adenosquamous carcinoma group(c), small cell carcinoma group(d), large cell carcinoma group(e) and the normal group(f).The squamous cell carcinoma group consisted of the highly-differentiated group(a1), moderately-differentiated group(a2)and poorly-differentiated group(a3). The adenocarcinoma group consisted of the highly-differentiated group(b1), moderately-differentiated group(b2), poorly-differenti--ated group(b3) and the bronchilolalveolar carcinoma group(b4). Three or fourviews were selected stochastically from each stain which was cut from three paraffin masses selected randomly and stained by Hematoxylin-Eosin. We measured the nucleus and carcinomatous cell using the Image-Pro analytical software. With the carcinomatous cell as the reference space, the following parameters of the nuclear were measured according to the stereologic formulas. The parameters were the volume density(Vvn), surface density(Svn), ratio of surface to volume(Rsvn), numerical density(Nvn), mean volume(vn), mean surface(sn), ratio of nuclear to plasm(Rnp), mean free distance( n) . And with the nuclear as the refrence space, the nucleolus parameter of the Nvnu was measured. The parameters were compared to analyse the differences among different groups.The correlations were done between the parameters showing significant difference among different graded squamous cell carcinomas and the differentiation of it. The following groups were separately submitted to discriminant analysis and stepwise discriminant analysis.They were the different subtypes of the lung cancer and the normal group, the different graded squamous cell carcinoma, adenocarcinoma and the normal group, and the poorly-differentiated lung cancer consisted of the poorly-differentiated squamous cell carcinoma, the poorly-differentiated adenocarcinoma, the small cell carcinoma and the large cell carcinoma.A total of 24 fresh lung carcinomatous specimens and normal lung mucosas from the lung surgical resections and fiberbronchoscope were collected. The views were photographed stochastically in the electron microscopy according to the stereological method and the test was done using the testing system of the square gridding made by the Image-Pro analytical software. With the carcinomatous cells as the reference space of the nuclear, the plasm as the reference space of the mitochondrion and lysosome, and the nuclear as the reference of the nucleolus, their parameters of volume density (Vv), surface density (Sv), numerical density (Nv), mean volume(v), mean surface(s), ratio of nuclear to plasm(Rnp), mean free distance( ) and ratio of surface to volume Rsv were acquired according to the stereologic formulas. Their differencesamong different groups were compared. In addition, the intracellular microcyst in the lung adenocarcinoma were observed and analysed.Results:1. The basal measuring results of the nuclear stereologic parameters in the lung carcinomaThe seven parameters of Vvn, Svn, Rsvn, Nvn, vn, sn, and n showed significant difference between the small cell carcinoma and the squamous cell carcinoma, adenocarcinoma, adenosquamous carcinoma, and the large cell carcinoma. Among the four non-small... |
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