| Objective: To study the actions of matrix metalloprotienase- 2,-9(MMP-2,-9) in the devolopment of chronic glomerular disease; Evaluate the diagnose value of blood MMP-2,-9 concentration of in the renal fibrosis.Methods: 30 chronic glomerular disease patients were cut into proliferation group (14 case) and fibrosis group (16 case including glomerulosclerosis) accroding to renal fibrosis degree in the renal pathology. Setting another 6 cases health human as control group. The serum sample of all research objects were gotten to measure the serum MMP-2 and MMP-9 concentration with the method of ELISA; and all research objects were taken renal biopsy to measure the MMP-2 and MMP-9 expression in the renal tissue with the method of S-P, and made half quantitative analyse. Then compare the serum MMP-2 and MMP-9 level and MMP-2,-9 expression in the renal tissue among evrey group, and make sure if blood MMP-2 and MMP-9 level will be related to its expression in the renal tissue. Result: the serum MMP-2 level is no striking difference compared amomg every group. MMP-2 is mainly expressed in the renal tubular epithelial cell plasm in the pathology tissue, and its expression is obviously higher in the proliferation group and fibrosis group than in the normal control group, there is significant difference. But to compare between the proliferation group and fibrosis group , there is no difference.The serum MMP-9 level is strikingly higher in the proliferation group and fibrosis group than in the normal control group. But there is no difference between the two groups. The MMP-9 expression is very high in tubular epithelial cells, and its expression is strikingly different compared among every group.The serum MMP-2 , MMP-9 concentration and its expression in the renal tissue is positive correlation.Conclusion: Serum MMP-9 level increase and is positive correlation to the fibrosis degree in chronic renal glomerular disease. The MMP-9 expression increase in chronic renal glomerular disease, but this increased expression begin to decrease accompany with the fibrosis degree increased. Discussion: ECM continue to accumulate is the chief pathologic alteration on the renal fibrosis, its mechanism is very complicated, but to sum it up, it is that the kidney inherent cells synthesis and excrete ECM increase and its degradation process being inhibited. ECM are made from great molecul, in vivo, it surround the cells. The matrix are made up of collagen, proteoglycan, mucoprotein and elastic fiber and such ultra-molecul.In the past, people think the ECM as just a carriage without other actions, its function is supporting the tissue, just like the buildings made up of steel channel. But now, people begin to realize that the ECM and the cells have continued to carry out information convection pass through the cytokine and adhesiveness molecul recipient. The cells on the matrix begin to growth, cleavage, excrete products and differentiation movement, but the cells without linked to the ECM usually be in recuperate and arrest growth stage.The cytokine and enzyme being excreted from the inherent cells and foreign cells beside the matrix take a decisive actions on the ECM synthesis and degradation. Just because of this interaction, the ECM synthesis and degradation are in a kind of dynamic equilibrium which renew and metabolism very fast. Various kinds virulence factor take a different role in this ECM synthesis and degradation equilibrium by the means of affecting inherent cells excrete cytokine and enzyme and some other protein. Thus make ECM synthesis increase and degragation decrease. Therefore renal fibrosis slowly formed.The normal renal inherent cells excret trace MMP-9, it can make IV collagen releas solubitwlity hydroxy amino acid to maintain integra basement membrance. In this experiment, we observed that normal human being renal tubular epithelial cell express trace MMP-9, but in the renal glomerulus, the its expression even few. Glomerulus visceral layer epithelial cell can be seen by chan... |
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