Expression Of Costimulatory Molecules And Lymphocyte Subsets On Peripheral Lymphocytes In Patients With Idiopathic Thrombocytopenic Purura

Objective: To investigate the expression of costimulatory molecules and lymphocyte subsets on peripheral lymphocytes in patients with idiopathic thrombocytopenic purura(ITP) in order to research the pathogenesis of IIP. Methods: The expression of costimulatory molecules and lymphocyte subsets on the peripheral lymphocytes was measured by immunofluorescence and flow cytometry in 28 ITP patients and 15 normal subjects .The expression of PAIgG of ITP patients was analysed by ELISA method. Results: The expression of costimulatory molecules of CD28 and CD80 on the surface of peripheral lymphocytes was normal in ITP patients. Compared with normal controls, the expression of costimulatory molecule CD86 increased in ITP patients (p<0. 01).The expression of CD19+ was higher in ITP patients than in normal controls(p<0. 01), but the expression of CD3+, CD3+CD4+ CD3+CD4+/CD3+CD8+, CDS-CD16+CD56+ (NK cell) was lower in ITP patients than in normal controls (P<0. 05). The PAIgG level was higher in 21 ITP cases with a mean of (197. 39 + 67. 81) ng/107 pit. A positive correlation was found between the expression of CD86 and the number of megakaryocyte (r= 0. 20, p<0. 05) . The expression of CD19+ had positive correlation with the onset time (r= 0.62, p=0. 01) and the number of megakaryocyte (r=0. 61, p= 0.01) ,while the expression of CD3+CD4+ had negative correlation with the onset time (r=-0.51 , p=0. 04). We also observed that there was negative correlation between the expression of NK cells and the number of megakaryocyte (r=-0. 55, p= 0. 03) . Conclusions: Most of lymphocyte subsets expression in ITP patients were abnormal and the disorder of the cellular immunity , humoral immunity function and NK cells is involved in pathogenesis of ITP . The expression of CD28 and CD80 on the surface of peripheral lymphocytes in ITP patients had no deficiency, but the expression of CD86 on the surface of peripheral lymphocytes in ITPpatients increased and it may be related to the pathogenesis of IIP. Our research may provide a new way in the treatment of IIP with monoclonal antibody or regulating lymphocyte subsets.Postgraduate Zhang chunmei (clinical hematology) Directed by Prof.Zhao chunting , Prof. Teng qingliang...