The Content Of Resveratrol, Piceid In Polygonum Cuspidatum And Resveratrol In Arachis Hypogaea

Resveratrol is a kind of phytoalexin, which is low molecular weight, biologically active metabolite produced by plants as defense to various exogenous stimuli. Piceid is the glycoside of resveratrol. Research was done to determine the content of reveratrol and piceid in Polygonum cuspidatum and the content of reveratrol in the different tissues and different processing samples of peanut.Reveratrol and piceid were extracted from P.cuspidatum with 95% ethanol and the content was determined by HPLC (high performance liquid chromatographic), with ODS Hypersil column (4.6mmx250.0mm, 5um). A mixture of CH3CN/H2O (20/80) was used as the mobile phase and the wavelength for detecting was 306nm. Results indicated that the linear range of resveratrol was 0.01168 ~ 0.05840mg/mL(r=0.9999, a =0.01).The average content of revesratrol was 0.441%, and the average recovery rate was 98.33%; The linear range of piceid was 0.0216~ 0.1944mg/mL(r=0.9999, a =0.01).The average content of piceid was 1.756%, and the average recovery rate was 98.46%.Reveratrol was extracted from the different tissues and different processing samples of peanut with 95% ethanol. The extract was purified by chromatographic (the mixture of AI2O3 and silica gel as solid phase, 80% ethanol as mobile phase).Then the content was determined by HPLC, with ODS Hypersil column (4.6mmx250.0mm, 5um). A mixture of CH3OH/H2O (45/55) was used as the mobile phase and the wavelength for detecting was 306nm. Result indicated that the linear range was 0.01184~0.05920mg/mL(r=0.9999, a =0.01).The root of Arachis hypogaea contained the highest content of resveratrol (0.1795mg/g). The average recovery rate was 97.98%. The stem, shell and boiled peanut contained very little resveratrol. The leaf, seed, toasted peanut and peanut butter did not contain resveratrol.