The Effect Of Apolipoprotein E Polmorphism On Coronary Heart Disease Therapy That Were Lipid-regulated And Atherosclerotic Plaques Eliminated By Atorvastatin

BACKGROUND AND OBJECTIVE:Recently,studies have show that gene type of individual decided susceptibility on environment factor .Genetic factors play an important role in serum lipid metabolism, apolipoprotein E (apoE) polmorphism have relativity with susceptibility to Coronary heart Disease (CHD) in defferent gene type as well as to CHD severity and level of lipid metabolism Polymerase chain eaction-restricted fragment length polmorphism was used to test 121 patients gene type with CHD .To explore the effect of apolipoprotein E Polmorphism on Coronary heart Disease Therapy That were Lipid-regulated and atherosclerotic Plaques .To investigate the assosiations between apolipoprotein E (apoE) polmorphism and coronary heart disease(CHD) by examine patient ' s apoE genes.To explore the new direction in Cardiology and provide new methods on preventing (1) To investigate the assosiations between apolipoprotein E (apoE) polmorphism and coronary heart disease(CHD) by examine patient' s apoE genes.(2)Respectively to check serum lipid of patient with CHD thatwere givened atorvastation before and half a year later, at the same time,to analyze the effecter of apoE polmorphism on lipid-regulating therpy. (3)To explore the role that apoE polmorphism affected the effect in coronary atherosclerotic plaque retard by atorvastation.METHODS: (1) Using Polymerase chain reaction -restricted fragment length polmorphism (PCR-RELP),measuring 121 patients'genes with CHD that was in Department of Cardiovasology ,Xijing hospital and analyzing assosiation between apoE polmorphism and CHD. (2) 95patients were given atorvastatin 10mg day-1 and respectively check plasma Lipid levels before and 6 months later,to observe the efficacy of apoE polmorphism on lipid-regulating. (3)Before 33patients were given atorvastatin 10mg day-1 and 6 months Later,they all underwent CAG (Coronary Angiography), survey coronary atherosclerotic plaque retard and pertinence with ApoE polmorphism by QCA (Quantitative Coronary Angiography,)RESULTS: (1) apoE genotype distributing:all patient' s apoE fragment were amplified by PCR satisfaction.specical fragment were 233bp.5 genotypes were found. They were genotype E2/2(2cases) E3/2 (18 cases) E3/3 (52 cases) E3/4 ( 44 cases) E4/4( 5 cases). But genotype E2/4 was not found. 5 genotypes were mergered 3 groups. They were E2/2+ E3/2 E3/3 and E3/4+E4/4 groups respectively.The alleles frequencies were 17% 43% and40% respectively .Among them,early onset CHD group with E4 Alleles frequencies < 40%) was higer than aged group (9%) .E3 was lower (17%vs35%) Significant difference were observed between 2 groups (P<0.01) .Patient with E2/2 and E3/2 were fewer,so they were not analysed. (2)ApoE genotype correlation factor. Early-onset CHD ratio in genotype E3/4+E4/4 is lower (P<0.05) and there is no significant difference between three groups in smoking franks,butfrequence of multiatherosclerotic plaques were significantly higher (P<0.05) .In E3/4+E4/4 group,TC and LDLch were higher than other genotype (P<0.01) . there was no significant difference on TG and HDLc in each genotyoe. (3) ApoE polmorphism was asscosiation on lipid-regulating of Atorvastatin. To compare E3/4+E4/4 genotype group ,the levels of TC and LDLch were receder and it was significant difference.but no significant difference on HDLc highten and TG depressed. (4) After patients were given Atorvastatin and 6 months later,coronary atherosclerotic plaques therapy was association with genotype.there was significant difference between each groups.But it was no significant difference in each group.CONCLUTION: (1)ApoE polmorphism was associated with more extensive in early-onset CHD and levels of serum lipid. (2) ApoE polmorphism was also associated lipid-regulating and coronary atherosclerotic plaque therpy. (3)During patients with CHD and persons with CHD risk factors,Measuring ApoE genotype ,these methods can provide evidences of CHD in earlier period,also can monitor patient' s condition and prognosis (4)The result demonstrate that atorvastatin had the role of re...