| PrefaceClinical repair of trauma or operation induced peripheral nerve defect is one of the unsolved surgical problems for years. If the nerve defect cannot be recovered by surgical suture,nerve graft is needed for the nerve regeneration, which can repair this defect. For years, nerve defect is repaired mainly by autologous nerve graft that is limited by the insufficient source. Although great effort has been made in the experimental study by using nerve heterograft and some advance has been achieved, antigenicity and immunological rejection still remain unsolved, which have not shown breakthrough yet. Recently, researchers at home and abroad have noted that muscle bridge can induce nerve regeneration. Unfortunately, it is still not available in clinical practice due to the unsatisfactory effect . Many factors nay be responsible for the poor lutcome, but at least the fact that connective tissue outgrows the nerve regeneration should be considered as one of the key factors. For that reason, our experiment employed bFGF for promoting Schwann cell proliferation and axon growth as well as vitamin C - lowering agent for decreasing collagen production by fibroblast. Our degradable compound membrane containing both bFGF and vitamin C - lowering agent can enhance nerve regeneration and on the other hand it can lessen or inhibit connective tissue proliferation. Therefore, nerve regeneration of muscle bridge is greatly improved.Materials1. Animals: 32 healthy big - ear male and female white rabbits in animal breeding center of CMU, weighing 2500 - 3000 g.2. Main reagents:bFGF complex degradation membrane; release from chemical research a-gency in Chengdu.3. Main instruments: image analysis device :Leca DMLB electron microscope :H -7100 Hitachi microscope: Olympus BH2 - RFC A electromyographic deviceMethods1. 64 median nerves of 32 rabbits (right and left legs) were divided into 2 groups, cut median nerves 2. 5cm length out in the same section and use sartori-us muscle for muscular bridge material in the same condition. A group use bFGF composite degradation membranetoencapsulatethelocation that is sewed by muscular bridge, it is experimental group. There is no bFGF composite degradation membrane in B group, it is control group. At different periods after the operation , cut the bridged sections, make into sepecimens which are observed by light- microscop and electricity - microscope and determinated by image - analyzing instrument, also hydroxyproline and adhesion quantitation are measured.2. Statistical significane was calculated by T test and analysis of variance by STATISTICA5.0 software.Results1. Visual observation; the bridged segment in group A was smoother and thicker than that in group B, and the suture site showed slighter enlargement and continuousness with connective tissue.2. Microscope observation; osmate and weil dyeing showed that the amount of sheathed fiber in group A was larger than that in group B, and the thickness of the fiber is thicker in grouo A than that in group B. Under electron microscope, the myelinated nerve fiber is thicker, and the lamina is more apparentand stained deeply in the proximal segment than that in the distal. By comparison allthe myelinated nerve fiber in the distal,we find that A group has a thicker mye-lin sheath, more evident and deep - stained lamina than B group.3. Image analysis: 180 days after operation, the amount of nerve fiber at proximal and distal segment in group A is significantly higher than that in group B(P<0. 001). Which suggested that Bfgf may promote regeneration of nerve. The study about the diameter of sheathed nerve fiber 180 days after operation also showed that bFGF could promote the development of nerve fiber.4. Adhesive quantitive test: 90 days after operation the adhesive degree in group A is significantly lighter than that in group B( P <0.01).5. Hydroxyproline quantitive test; 90 days after operation the hydroxypro-line degree in group A is significantly lighter than that in group B... |
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