| Objective TO create the rat model of furazolidone-induced dilated cardiomyopathy (Fz-DCM) and explore the characterization of ventricular remodeling in Fz-DCMMethods sixty wistar rats were randomized into the DCM group and the control group, and then each group was divided into three subgroups bas on the feeding time, netmely the four-week, eight-week and twelve-week subgroup wich has ten rats equally. Each subgroup of the DCM group was fed with furazolidone for four weeks, eight weeks and twelve weeks respectively, the control group was fed regulately, and then left ventricular dimension and cardiac funtion were detected by echocardiogram. Aortic and right atrial pressure were measured by invasive catheter. Left ventricular interior diameter and the thickness of left ventricular free wall were measured after the rats were killed. Myocardial collagen network remodeling was observed by Van Gieson stain and collagen volumefraction (CVF) was caculated by pathalogical imagine analysis system. Result (1)The total incidence rate of DCM was 66.6%(20/30) inDCM group fed with furazolidone,among them the quantity of the rats diagnosised as DCM by histopathology was 8(8/10),6(6/10) and 6(6/10) in the four-week , eight-week and twelve-week subgroup respectively.(2)The left ventricular end-diastolic diameter (LVED) and left ventricular end-systolic diameter(LVES) in the four-week and eigh-week subgroups of the DCM group were larger than those in the corresponding subgroups of the cntrol group[LVED(cm): 0.59 ± 0.41VS 0.52 ± 0.29 p<0.01l, 0.68 ± 0.02 VS 0.63 ± 0.46 p<0.01 ; LVES(cm): 0.35±0.04 VS 0.29±0.03 p<0.01, 0.46 ± 0.05 VS 0.35 ± 0.29 p<0.01],however,there were no differences between the two eighth-week subgroups in LVED and LVES ( all p>0.05) . (3) Compared with the corresponding subgroups of control group ,the fraction shortening[FS(%)] in all subgroups of the DCM group was decreased significantly (38.46±3.92 VS 46.71 ± 3.50, p<0.01, 29.62 ± 4.09 VS 36.46 ± 2.56 P<0.05 , 30.97 ± 4.97 VS 44.44 ± 3.26 p<0.01 ),moreover, the left ventricular ejection fraction[LVEF(%)] in all subgroups of the DCM group was decreased significantly. (75.71 ± 3.62 VS 83.20±3.34 , 62.76 ± 6.12 VS 71.46 ± 1.15, 63.82±7.24 VS 80.96 ± 3.16 all p<0.01). (4) The right atrial pressure(mmHg) in all subgroups of the DCM group increased more significantly than those of the corresponding subgroups of the cntrol group (13.90 ±4.91 VS 4.20±3.27, 19.67 ± 2.94 VS 6.70±2.95, 11.25 ± 3.28 VS 5.70 ± 2.00 all p<0.01),but there was no difference between two groups in the aortic pressure in all corresponding subgroups(all P>0.05). (5) The left ventricular interior diameter(mm) in all subgroups of the DCM group was wider than those of the corresponding subgroups of the control group (4.25 ± 0.60 VS 3.08 ± 0.92 P<0.05 , 4.67 ± 0.61 VS 3.70 ± 0.59 p<0.01, 4.37 ± 0.64 VS 3.45 ± 0.37 p<0.01),and the thicknes of left ventricular free wall(mm) in the eight-week and twelve-week subgroups of the DCM group became thinner significantly than those of the corresponding subgroups of the control group (2.58 ± 0.38 VS 4.00 ± 0.58 p<0.01,3.13 ± 0.23 VS 3.55 ± 0.50 P<0.05), but there was no difference in two corresponding fourth-week subgroups (P>0.05) . (6)Compared with the corresponding subgroups of the control group, the ratio of left ventricle weight and body weight(mg/g) was increased significantly in the twelve-week subgroup.( 2.98 ± 0.15 VS 2.76 ± 0.10 F<0.05) ,but no differene of the ratio of left ventriculal weight and heart weight (mg/mg)was found in all subgroups of two groups (all P>0.05) .(7) Cardiac myocyte hypertrophy accompanied with nucleus augmentation,differentiation and defferiation . There were spot lysis, granular degeneration and vacuolation in themyocytic plasm. The intercellular space became enlargment. Those were observed in the rat myocardium in all sample; in the DCM group, but not occurried in the control group. |
PDF Full Text Request