Inhibitory Effect Of EGFR Antisense Oligodeoxynucleotide On Human Hepatoma Cell Line

Objective: to investigate the effects of antisense oligodeoxynucleotide targeting against epidermal growth factor receptor (EGFR) on the human hepatoma cell line SMMC-7721 and to provide a new target for gene therapy for hepatoma.Methods:method of MTT was adopted to determine the proliferation of tumor cell in vitro and SMMC-7721 cell with or without pretreatment was inoculated into the nude mice in vive.Results: AS-ODN acted as a specific growth inhibitor on SMMC-7721 with a sequence specific and time-dependent manner. After 24h,48h,72h,the inhibitory rate of proliferation of SMMC-7721 cell treated EGFR AS-ODN was 8%,32%,34%,respectively and tumorigenic rate significantly reduced (7/7,6/7,1/7 respectively p<0.05).Conclusion: EGFR AS-ODN could significantly inhibit the proliferation of human hepatoma cell in vitro as well as in vivo,indicating EGFR may play an important role the hepatoma and be a new target of treatment to hepatoma.